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环境病理学,毒理学和肿瘤学期刊
影响因子: 1.625 5年影响因子: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN 打印: 0731-8898
ISSN 在线: 2162-6537

环境病理学,毒理学和肿瘤学期刊

DOI: 10.1615/JEnvironPatholToxicolOncol.2019027318
pages 185-194

AgNPs from Nigella sativa Control Breast Cancer: An In Vitro Study

Baradhan Rohini
School of Life Sciences, BS Abdur Rahman Crescent Institute of Science & Technology, Chennai, India
Tahira Akther
School of Life Sciences, BS Abdur Rahman Crescent Institute of Science & Technology, Chennai, India
Mohammad Waseem
School of Life Sciences, BS Abdur Rahman Crescent Institute of Science & Technology, Chennai, India
Jasim Khan
Department of Toxicology, JamiaHamdard, New Delhi, India
Mohammad Kashif
Department of Toxicology, JamiaHamdard, New Delhi, India
Srinivasan Hemalatha
School of Life Sciences, BS Abdur Rahman Crescent Institute of Science & Technology, Chennai, India

ABSTRACT

In our current study, we synthesized silver nanoparticles (AgNPs) from an aqueous seed extract of Nigella sativa. The seed extract contains phytochemical compounds including phenols, terpenoids, and flavonoids that may act as reducing agents and are able to convert metal ions to metal nanoparticles. The formation of synthesized AgNPs was characterized using UV-visible spectroscopy, Fourier transform infra-red spectroscopy (FT-IR), scanning electron microscopy (SEM) and energy dispersive analysis of X-rays (EDX). The efficacy of N-AgNPs against human breast cancer (MCF-7) cells was tested. The synthesized AgNPs displayed dose-dependent cytotoxicity (1−200 µg/mL) against MCF-7 cells. Morphological alterations of the cells also appeared as bright field images. Treatment of synthesized AgNPs altered the expression of Bax and Bcl-2 (apoptotic proteins) and COX-2 (inflammatory marker) in MCF-7 cells. To our knowledge, this is the first report demonstrating that N-AgNPs from Nigella sativa can induce apoptosis in MCF-7 cells.

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