图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
环境病理学,毒理学和肿瘤学期刊
影响因子: 1.625 5年影响因子: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN 打印: 0731-8898
ISSN 在线: 2162-6537

环境病理学,毒理学和肿瘤学期刊

DOI: 10.1615/JEnvironPatholToxicolOncol.v21.i2.50
9 pages

Oxidant-Sensitive Transcription Factor and Cyclooxygenase-2 by Helicobacter pylori Stimulation in Human Gastric Cancer Cells

Hyeyoung Kim
Department of Pharmacology and Institute ofGastroen-terology, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Korea
Joo Weon Lim
Department of Pharmacology and Institute ofGastroen-terology, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Korea
Jeong Yeon Seo
Department of Pharmacology and Institute ofGastroen-terology, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Korea
Kyung Hwan Kim
Department of Pharmacology and Institute ofGastroen-terology, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Korea

ABSTRACT

Helicobacter pylori (H. pylori) infection might activate nuclear factor-kB (NF-kB), an oxidant-sensitive transcription regulator of inducible expression of inflammatory genes such as cyclooxygenase-2 (COX-2). We studied the role of NF-kB on expression of COX-2 in H. pylori-stimulated gastric cancer cell lines by using antioxidants, glutathione (GSH), and N-acetylcysteine (NAC) as well as an NF-kB inhibitor, pyrrolidine dithiocarbamate (PDTC). Gastric adenocarcinoma cell lines derived from Caucasian (AGS) cells and Korean (SNU-484) cells were used to study the role of NF-kB on COX-2 expression by H. pylori. They were treated with GSH, NAC, or PDTC in the presence of H. pylori. mRNA expression and protein level for COX-2 were determined by Northern blot and RT-PCR analysis as well as Western blot analysis. NF-kB activation was examined by electrophoretic mobility shift assay. As a result, H. pylori induced a time-dependent expression of mRNA and protein for COX-2 via activation of NF-kB, which was inhibited by GSH, NAC, and PDTC in the cells. In conclusion, oxidant-sensitive transcription factor NF-kB may play a novel role in expression of COX-2 by H. pylori stimulation in gastric cancer cells.


Articles with similar content:

The Role of Nitric Oxide and Cyclooxygenase-2 in Attenuating Apoptosis
Journal of Environmental Pathology, Toxicology and Oncology, Vol.21, 2002, issue 2
Andreas von Knethen, Bernhard Brune
In Vitro Evidence of the Role of COX-2 in Attenuating Gastric Inflammation and Promoting Gastric Carcinogenesis
Journal of Environmental Pathology, Toxicology and Oncology, Vol.21, 2002, issue 2
Seonghyang Sohn, Hyuk-Jae Kwon, Young-Joon Surh, Ki-Baik Hahm, Young-Bae Kim, Ki-Myung Lee, Hee-Jae Joo, Seung-Won Cho, Jeong-Sang Lee, Ho-Yeong Lim, Won-Seok Kim
Suppressive Effect of Natural Sesquiterpenoids on Inducible Cyclooxygenase (COX-2) and Nitric Oxide Synthase (iNOS) Activity in Mouse Macrophage Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.21, 2002, issue 2
Won-Yoon Chung, O-Jin Oh, Chai-Hee Hong, Jae-Kwan Hwang, Sun-Sook Kim, Sang Kook Lee, Sun-Kyung Huh, Kwang-Kyun Park, Hye-Young Min
Effects of Yakuchinone A and Yakuchinone В on the Phorbol Ester-Induced Expression of COX-2 and iNOS and Activation of NF-kB in Mouse Skin
Journal of Environmental Pathology, Toxicology and Oncology, Vol.21, 2002, issue 2
Young-Joon Surh, Ok Hee Kim, Jee-Young Kang, Hoil Kang, Kyung-Soo Chun
Role of Raf Kinase Inhibitor Protein in Helicobacter pylori-Mediated Signaling in Gastric Cancer
Critical Reviews™ in Oncogenesis, Vol.19, 2014, issue 6
Devasis Chatterjee, Sam Cross-Knorr, Steven F. Moss, Arlin B. Rogers, Sicheng Wen, Liana Nisimova