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环境病理学,毒理学和肿瘤学期刊
影响因子: 1.625 5年影响因子: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN 打印: 0731-8898
ISSN 在线: 2162-6537

环境病理学,毒理学和肿瘤学期刊

DOI: 10.1615/JEnvironPatholToxicolOncol.v30.i4.60
pages 333-340

Bystander Effect of Conditioned Medium from Low and High Doses of γ-irradiated Human Leukemic Cells on Normal Lymphocytes and Cancer Cells

Badri Narain Pandey
Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, India
Amit Kumar
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India
Manjoor Ali
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai - 400085, India
Kaushala Prasad Mishra
Department of Life Sciences, University of Mumbai, Mumbai, India; Nehru Gram Bharati University, Allahabad, UP, India; Foundation for Education and Research, India and BM International Research Centre, Mumbai, India

ABSTRACT

The present study investigated the effect of irradiated conditioned medium (ICM), obtained from human promyelocytic leukemic cells (HL-60) γ-irradiated either with low-dose (5 cGy; ICM-L) or high-dose radiation (1 Gy; ICM-H) on unirradiated HL-60 and normal human blood lymphocytes. A dose-dependent increase in apoptosis was observed in HL-60 cells cultured in ICM obtained after different doses of irradiation (0.5−10 cGy), which showed saturation in ICM from 5 cGy. Furthermore, ICM-L and ICM-H induced differential apoptotic responses in unirradiated HL-60 cells and lymphocytes. Apoptosis in lymphocytes cultured in ICM-H was significantly higher than when cells were cultured in unirradiated conditioned medium or ICM-L. Furthermore, induction of apoptosis in bystander HL-60 cells and lymphocytes cultured in ICM-L/ICM-H seems to be associated with an increase in intracellular reactive oxygen species (ROSs) and alterations in mitochondrial membrane potential. These results suggest that differential bystander responses from irradiated cancer cells toward cancer and normal cells depending on the applied dose and/or dose rate of radiation..


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