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环境病理学,毒理学和肿瘤学期刊
影响因子: 1.625 5年影响因子: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN 打印: 0731-8898
ISSN 在线: 2162-6537

环境病理学,毒理学和肿瘤学期刊

DOI: 10.1615/JEnvironPatholToxicolOncol.2013007541
pages 157-163

MNU-Induced Rat Mammary Carcinomas: Immunohistology and Estrogen Receptor Expression

R. Soares-Maia
Abel Salazar Institute for Biomedical Sciences, University of Porto, Porto, Portugal
A. I. Faustino-Rocha
Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro, Vila Real, Portugal
C. I. Teixeira-Guedes
Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro, Vila Real, Portugal
J. Pinho-Oliveira
Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro, Vila Real, Portugal
D. Talhada
Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro, Vila Real, Portugal
A. Rema
Department of Pathology and Molecular Immunology, Abel Salazar Institute for Biomedical Sciences, University of Porto, Porto, Portugal
F. Faria
Department of Pathology and Molecular Immunology, Abel Salazar Institute for Biomedical Sciences, University of Porto, Porto, Portugal
M. Ginja
Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro, Vila Real; Center for the Reasearch and Technology of Agro-Environmental and Biological Sciences, University of Tras-os-Montes and Alto Douro, Vila Real, Portugal
R. Ferreira
Organic Chemistry of Natural Products and Agrifood, Mass Spectrometry Center, Department of Chemistry, University of Aveiro, Campus Universitario de Santiago, Aveiro, Portugal
R. M. Gil da Costa
Laboratory for Process, Environmental and Energy Engineering, Faculty of Engineering, University of Porto; Faculty of Veterinary Medicine, Lusophonous University of Humanities and Technologies, Lisboa, Portugal
Paula A. Oliveira
Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro, Vila Real; Center for the Study of Animal Sciences, School of Agriculture and Veterinary Sciences, University of Tras-os-Montes and Alto Douro, Vila Real, Portugal
C. Lopes
Portuguese Institute of Oncology, Porto Research Center, Portuguese Institute of Oncology, Porto, Portugal

ABSTRACT

Environmental exposure to nitrosamines is associated with the development of cancer in a variety of target organs. One such carcinogen, N-methyl-N-nitrosurea (MNU), has long been used to induce mammary tumors in rats, which provide a useful model to study mammary carcinogenesis. However, some poorly clarified issues remain, such as the lack of a clear description of morphological patterns of tumors and the distribution and role of estrogen receptors (ERs) during tumor progression, as tumors overexpressing ERs show a paradoxical tendency to recur after ovariectomy.
Mammary carcinomas were induced in Sprague-Dawley rats using MNU. The tumors were studied histologically and distribution of smooth muscle actin and ERs was studied immunohistochemically. All tumors presented both an epithelial and a myoepithelial component, demonstrated by immunohistochemical detection of smooth muscle actin. Tumors showed distinct histological patterns: well-differentiated papillary and adenoid areas and poorly differentiated solid and spindle-cell foci. Overexpression of ERs (>75% of labelled cells vs. 0−75% in control tissue) occurred in papillary and adenoid areas but not in solid and spindle-cell foci. Poorly differentiated tumor foci are likely to represent a more advanced, estrogen-independent phase in cancer progression and constitute the basis for tumor recurrence after ovariectomy.


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