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环境病理学,毒理学和肿瘤学期刊
影响因子: 1.241 5年影响因子: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN 打印: 0731-8898
ISSN 在线: 2162-6537

环境病理学,毒理学和肿瘤学期刊

DOI: 10.1615/JEnvPathToxOncol.v23.i2.30
10 pages

Cellular Inactivation and Chromosomal Aberrations: Initial Damage

Arnaud Boissiere
Groupe de Physique des Solides, Universites Paris 6 et Paris 7, Paris, France
Anne Eschenbrenner
Groupe de Physique des Solides, Universites Paris 6 et Paris 7, Paris, France
Francois Gobert
Groupe de Physique des Solides, Universites Paris 6 et Paris 7, Paris, France
Marie-Anne Herve du Penhoat
Groupe de Physique des Solides, Universites Paris 6 et Paris 7, Paris, France
Francois Abel
Groupe de Physique des Solides, Universites Paris 6 et Paris 7, Paris, France
Michele Lamoureux
Groupe de Physique des Solides, Universites Paris 6 et Paris 7, Paris, France
Luis Martins
Laboratoire de Radiobiologie et Oncologie, Fontenay aux Roses, France
Marie-Francoise Politis
Groupe de Physique des Solides, Universites Paris 6 et Paris 7, Paris, France
Michele Ricoul
Laboratoire de Radiobiologie et Oncologie, Fontenay aux Roses, France
Alain Touati
Groupe de Physique des Solides, Universites Paris 6 et Paris 7, Paris, France
Evelyne Sage
Laboratoire de Genotoxicite et Modulation de l'Expression Genique, Institut Curie Recherche, Orsay, France
Laure Sabatier
Laboratoire de Radiobiologie et Oncologie, Fontenay aux Roses, France
Annie Chetioui
Groupe de Physique des Solides, Universites Paris 6 et Paris 7, Paris, France

ABSTRACT

It has been proposed that unrepaired or misrepaired complex lesions of DNA are responsible for cell inactivation and chromosomal aberrations. The detailed features of the critical damage and the nature of initiating physical events are actively investigated. We studied the role of inner-shell (core) ionizations in DNA atoms is studied. Ultrasoft X-rays from LURE synchrotron radiation have been used to mimic core events induced by ionizing radiations. For biological matter, inner-shell photoionization is indeed the main interaction channel of these radiations. Moreover, by tuning the X-ray energy below and above the carbon K-threshold, it is possible to achieve a two-fold increase in the number of core-ionizations in DNA for a same dose. Cell survival and chromosome aberrations have thus been studied at three iso-attenuated energies: 250,350, and 810 eV. Relative biological efficiencies (RBEs) for cell inactivation and chromosome aberrations were found to be strongly correlated with the yields of core events in DNA.