图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
免疫学评论综述™
影响因子: 1.352 5年影响因子: 3.347 SJR: 0.657 SNIP: 0.55 CiteScore™: 2.19

ISSN 打印: 1040-8401
ISSN 在线: 2162-6472

免疫学评论综述™

DOI: 10.1615/CritRevImmunol.2018025938
pages 131-143

Measuring T Cell Responses by Flow Cytometry–Based Fluorescence In Situ Hybridization

J.J. Freen-van Heeren
Department of Hematopoiesis, Sanquin Research-AMC Landsteiner Laboratory, Amsterdam, The Netherlands
Benoit P. Nicolet
Department of Hematopoiesis, Sanquin Research-AMC Landsteiner Laboratory, Amsterdam, The Netherlands
Monika C. Wolkers
Department of Hematopoiesis, Sanquin Research-AMC Landsteiner Laboratory, Amsterdam, The Netherlands

ABSTRACT

T cells produce a wide variety of effector molecules in response to infections, such as cytokines, chemokines, granzymes, and perforins. Because different stimuli promote the production of specific effector molecules, T cell responses come in different flavors. In addition, single-cell analysis of protein production revealed that T cells respond heterogeneously to activation. To unravel the regulatory mechanisms that determine T cell effector function, novel methods were developed that simultaneously measure protein levels with the corresponding mRNA. These flow cytometry-based fluorescence in situ hybridization (Flow-FISH) technologies allow for multiparameter analysis with single-cell resolution of both nucleic acids and proteins. Here, we review the currently available methods of Flow-FISH and describe the possible applications thereof, with a specific focus on T cells.