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水生生物学期刊
SJR: 0.227 SNIP: 0.575 CiteScore™: 0.24

ISSN 打印: 0018-8166
ISSN 在线: 1943-5991

水生生物学期刊

DOI: 10.1615/HydrobJ.v38.i4.80
8 pages

An Optimized Method for Total Counting of Benthic Bacteria

A. N. Butorin
Institute of Biology of Inland Waters Russian Academy of Sciences Borok, Russia
A. N. Dzyuban
Institute of Biology of Inland Waters, Russian Academy of Sciences, Borok, Russia
A. Yu. Gorbneko
Institute of Biology of Inland Waters Russian Academy of Sciences Borok, Russia
A. I. Kuznetsova
Institute of Biology of Inland Waters Russian Academy of Sciences Borok, Russia

ABSTRACT

It is shown that numbers of benthic bacteria (BB) can be determined with a higher accuracy due to the method described. The procedure proposed by the authors consisted in the preceding inhibiting the ground consumers of BB with subsequent repeated catch and removal of microorganisms from the partitioned ground specimens. A more appropriative microscopic dye was also chosen, and the mathematical model interpreting the changes in quantities of the 'caught' BB was applied. Other methods employed in similar studies allow detecting only about 50% of the total number of BB.


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