图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
等离子医学
SJR: 0.278 SNIP: 0.183 CiteScore™: 0.57

ISSN 打印: 1947-5764
ISSN 在线: 1947-5772

等离子医学

DOI: 10.1615/PlasmaMed.2017019064
pages 187-200

Bactericidal Effect of a Dielectric Barrier Discharge Plasma Jet Generated in Laminar and Preturbulent Helium Flows

Olga Stepanova
Saint Petersburg State University, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia
Oksana Rybalchenko
Saint Petersburg State University, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia
Mikhail Pinchuk
Institute for Electrophysics and Electric Power of Russian Academy of Sciences, 18 Dvortsovaya nab., St. Petersburg, 191186, Russia
Alexander Astafiev
Saint Petersburg State University, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia
Olga Orlova
Saint Petersburg State University, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia
Valentin A. Spodobin
Institute for Electrophysics and Electric Power, Russian Academy of Sciences, 18 Dvortsovaya nab., 191186, St.-Petersburg, Russia
Anatoly A. Kudryavtsev
St. Petersburg State University, St. Petersburg, 199034, Russia; ITMO University, 49 Kronverkskiy Ave., St. Petersburg, 197101, Russia

ABSTRACT

The bactericidal effect of a helium dielectric barrier discharge plasma jet generated in laminar (at 3 m/s) and preturbulent (at 10 m/s) helium flows is considered in terms of average discharge power and distance of treatment of Escherichia coli cells grown on a nutrient agar surface in a Petri dish. Sizes of bacteria inhibition zones are estimated and morphological changes in ultrastructure of bacterial cells are revealed using electron microscopy. This study suggests that the gas flow regime is an important factor in the production of the helium plasma jet used to inhibit bacteria. We reveal the different responses of E. coli cells treated by the helium plasma jet generated in laminar and preturbulent gas flows. Numerous focal destructions appear in the cells after 2 min of treatment with the laminar gas flow regime. Along with these focal destructions, expansion of cytoplasm and a superhelix of nucleoids are revealed with the preturbulent regime. We explain differences in behavior of cell morphology under the plasma jet formed at different gas flow regimes by the different gas-dynamic conditions of mixing ambient air into the helium flow.


Articles with similar content:

ANALYSIS OF PLASMA JETS GENERATED IN GAS AND GAS-WATER TORCHES
High Temperature Material Processes: An International Quarterly of High-Technology Plasma Processes, Vol.11, 2007, issue 1
T. Kavka, A. Maslani, O. Chumak, V. Sember, Vladimir Kopecky
Osteogenic Induction of Human Mesenchymal Stem Cells by Cold Atmospheric Argon Plasma
Plasma Medicine, Vol.6, 2016, issue 2
Adil Shahzad Ahmed, Erik Antonsen, Joseph Chavarria, Kurt Johnson, Tyler Brenneman, Scott Rosenfeld
Comparison of Biological Effects on Human Keratinocytes Using Different Plasma Treatment Regimes
Plasma Medicine, Vol.3, 2013, issue 1-2
Ulrike Lindequist, Rene Bussiahn, Thomas von Woedtke, Susanne Strassenburg, Kristian Wende, Ute Greim, Beate Haertel
Air-Based Coaxial Dielectric Barrier Discharge Plasma Source for Pseudomonas aeruginosa Biofilm Eradication
Plasma Medicine, Vol.7, 2017, issue 1
Diana Grondona, Leandro Giuliani, Juliana Soler-Arango, Magali Xaubet, Graciela Brelles-Mariño
NATURE OF ARGON AND NITROGEN ELECTRIC ARC FLUCTUATIONS IN PLASMA TORCH
High Temperature Material Processes: An International Quarterly of High-Technology Plasma Processes, Vol.2, 1998, issue 1
A. Kaminska, Michel A. Dudeck