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ISSN 打印: 1947-5764

ISSN 在线: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Nonthermal Plasma Increases Expression of Wound Healing Related Genes in a Keratinocyte Cell Line

卷 3, 册 1-2, 2013, pp. 125-136
DOI: 10.1615/PlasmaMed.2014008540
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摘要

The application of physical plasma in medicine has great potential in wound healing. Due to the generation of reactive oxygen and nitrogen species (ROS, RNS), emission of UV radiation and the generated electric fields can be used to stimulate epithelial and immune cells. To understand the processes on a molecular level the human keratinocyte cell line HaCaT was treated with a nonthermal atmospheric pressure argon plasma jet (kinpen). Subsequently, cellular RNA was isolated to conduct a quantitative polymerase chain reaction (qPCR) to monitor the magnitude of translation of genes related to wound healing. Plasma treatment induced an upregulation of vascular endothelial growth factor-a (VEGF-A), heparin-binding epidermal growth factor (EGF)-like growth factor (HBEGF), granulocyte macrophage colony-stimulating factor (GM-CSF), prostaglandin-endoperoxide synthase 2 (PTGS2) and interleukin-6 (IL-6) at the mRNA levels. This is a very promising result as the corresponding proteins are likely to be secreted and promote the wound healing process. Therefore plasma potentially induces the secretion of certain cytokines and growth factors, and hence, it could be the stimulus which is necessary to induce chronic wounds to heal.

对本文的引用
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