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等离子医学
SJR: 0.278 SNIP: 0.183 CiteScore™: 0.57

ISSN 打印: 1947-5764
ISSN 在线: 1947-5772

等离子医学

DOI: 10.1615/PlasmaMed.2018028269
pages 225-236

Hydra as a Model for Screening Ecotoxicological Effects of Plasma-Treated Water

Dana Ziuzina
School of Food Science and Environmental Health, Environmental Sustainability and Health Institute, Dublin Institute of Technology, Dublin 1, Ireland, UK
C. Sarangapani
School of Food Science and Environmental Health, Environmental Sustainability and Health Institute, Dublin Institute of Technology, Dublin 1, Ireland, UK
D. Boehm
School of Food Science and Environmental Health, Environmental Sustainability and Health Institute, Dublin Institute of Technology, Dublin 1, Ireland, UK
P. Bourke
School of Food Science and Environmental Health, Environmental Sustainability and Health Institute, Dublin Institute of Technology, Dublin 1, Ireland, UK

ABSTRACT

Atmospheric cold plasma (ACP) has been widely researched to generate function-alized solutions for decontamination of liquids and wastewater treatment. The focus of this work is the study of the ecotoxicological effects of plasma-treated water (PTW) using a free-living brown Hydra as an important and prevalent component of freshwater ecosystems. We obtained PTW by subjecting 25 mL of sterile deionized water to high-voltage (80 kV) contained ACP treatment for 15 (PTW15) and 25 (PTW25) min. With the use of a scoring procedure, we based toxicity measurements on progressive changes in Hydra morphology. The effects of PTW on Pseudomonas aeruginosa, Escherichia coli, and Bacillus atrophaeus cells were determined using a broth microdilution method that estimated minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results demonstrated that PTW is potentially toxic to biological systems. In Hydra tests, toxic effects were observed for PTW15 and PTW25 at concentrations above 50% and 25%, respectively. Similarly, PTW25 at a concentration of 25% is lethal for P. aeruginosa and E. coli, whereas an MBC of 37.5% was obtained for B. atrophaeus. The present investigation demonstrates that Hydra can be used as an additional in vivo tool to monitor the impact of plasma-processed solutions on the aquatic environment.