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国际生理学与病理学期刊
SJR: 0.116

ISSN 打印: 2155-014X
ISSN 在线: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

国际生理学与病理学期刊

DOI: 10.1615/IntJPhysPathophys.v8.i1.80
pages 77-89

Analysis of Association between 11 Single-Nucleotide Polymorphisms and Endothelium-Dependent Vasodilation in Children with Type 1 Diabetes Mellitus

Nataliya B. Pranik
Bogomolets National Medical University, Ministry of Health of Ukraine, Kyiv, Ukraine
Sergii V. Goncharov
Bogomoletz Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine
Veronika L. Gurianova
Bogomoletz Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine
V. G. Maidannik
Bogomolets National Medical University, Ministry of Health of Ukraine, Kyiv, Ukraine
Mykola V. Khaitovych
Bogomolets National Medical University, Kyiv, Ukraine
Olexiy O. Moibenko
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Viktor E. Dosenko
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

ABSTRACT

We studied the association between 11 single-nucleotide polymorphisms (SNPs) of 10 genes and the level of endothelium-dependent vasodilatation (EDVD) in 45 children suffering from type 1 diabetes mellitus. Polymorphisms G894→T of the eNOS exon 7 and T-786→C of the eNOS promoter, A1266→G of the Eln exon 16, T-381→C of the NPPB promoter, I\D of the ACE, Arg60→His of the LMP2, Met235→Thr of the AGT, A1166→C of the ATR1, C-1562→T of the MMP9, C-1306→T of the MMP2, and C-8→G of the PSMA6 were examined. It has been shown that children with genotypes G/T by eNOS (G894→T), G/G by Eln (A1266→G), C/C by NPPB (T-381→C) and I/I by ACE genes have lower EDVD (P < 0.05) compared to the patients with others allelic variants of these genes, and it is independent of the disease duration, the level of glycosylated hemoglobin, and baseline diameter of the brachial artery. The combination of the above genotypes most significantly affects EDVD decrease (r = 0.61; P < 0.01) compared to each separate genotype. Thus, the combined effect of genotypes G / T eNOS (G894→T), G / G Eln (A1266→G), C / C NPPB (T-381→C), and I / D ACE on endothelial function in children with type 1 diabetes mellitus is more significant than the duration of the disease and the level of HbAlc.