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国际生理学与病理学期刊
SJR: 0.106

ISSN 打印: 2155-014X
ISSN 在线: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

国际生理学与病理学期刊

DOI: 10.1615/IntJPhysPathophys.v8.i3.30
pages 219-229

Rising of Constitutive NO-Synthase Coupling and Hemoglobin Content in Erythrocytes of Old Animals by Iron- Containing Herbal Drug

S. I. Uretii
Bogomolets National Medical University, Ministry of Health of Ukraine, Kyiv, Ukraine
Anatoliy V. Kotsuruba
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Bogdan S. Kopyak
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

ABSTRACT

In in vivo experiments, we studied the effect of iron-containing drug (Urfuhem) on the content of hemoglobin (Hb) in the blood of old rats. To establish the biochemical mechanisms of the drug action, we determined the parameters of oxidative/nitrosative stress, hydrogen sulfide level (H2S) in plasma and erythrocytes, the level of non-heme iron in plasma, and erythrocyte sensitivity to acid hemolysis. It has been found that in old rats the drug significantly increases the Hb content in erythrocytes and reduces their resistance to acid hemolysis. After applying the drug, the rate of superoxide anion-radical (*O2-) generation in erythrocytes and the content of stable hydrogen peroxide (H2O2) both in plasma and erythrocytes, which in old animals far exceeded the reference value, were down-regulated. The drug did not reduce the high levels of hydroxyl radical (*OH) generation and excess NO de novo synthesis by iNOS in erythrocytes, but it reduced the pools of nitrate anion (NO3-) and its reutilization for NO synthesis. The drug introduction resulted in up-regulation of the rate of constitutive NO synthesis by cNOS in the plasma of old rats perhaps due to cNOS coupling. The results indicate that the reason for increasing proton (H+) permeability in erythrocytes of old rats that causes acid hemolysis can be the change in the balance between levels of oxidative and nitrosative stress in erythrocytes in favor of the latter. Thus, toxic *OH generation in the blood of old animals occurs due to formation and decomposition of peroxynitrite (ONOO-), not at the expense of the classical Fenton reaction in the presence of iron ions (Fe2+ ).


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