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SJR: 0.123

ISSN 打印: 2151-805X
ISSN 在线: 2151-8068


DOI: 10.1615/EthicsBiologyEngMed.2019030275
pages 59-76

Enter CRISPR: Jennifer Doudna's Autobiographical Assessment of the Science and Ethics of CRISPR/Cas9

Hub Zwart
Erasmus School of Philosophy, Erasmus University Rotterdam, P.O. Box 1738, Rotterdam, Zuid-Holland, Netherlands, 3000 DR


In 2012, Jennifer Doudna et al. published their landmark article on CRISPR/ Cas9. Five years later, Doudna published an autobiographical retrospective to come to terms with the "tsunami" of events that followed. The subtitle suggests that humans had acquired "unthinkable power" to refurbish life and deflect the course of evolution. Yet the subtitle of the prologue suggests a different view of human agency, seeing CRISPR as a technological pandemic, stressing our powerlessness to develop ethical and governance tools to contain the process. We seem overwhelmed by a surging biotechnological event. Science autobiographies constitute a fascinating genre, providing a window into the context of discovery, revealing what often remains unsaid in more formal academic publications. But they describe events from a decidedly personal and partisan perspective, wavering between self-analysis and self-justification, putting the individual frontstage, obfuscating how research is a collective endeavor. Doudna's memoir is analyzed from three perspectives: knowledge (CRISPR as a shift from reading to reediting genomes), power (memoirs as instruments in controversies over IPR), and ethics. Normative challenges allow researchers to constitute themselves as responsible subjects by developing new skills (bioethical deliberation) while calling forth new practices of the Self (writing science autobiographies). While traditional narrative suggests that, after an increase in dramatic tension, a period of equilibrium sets in, Doudna's retrospective voices the unsettling concern that we may lose control over the disruptive deflection we helped to bring about.


  1. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna J, Charpentier E. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012;337(6096):816-21. DOI: 10.1126/science.1225829.

  2. Doudna J, Sternberg S. A crack in creation: gene editing and the unthinkable power to control evolution. Boston: Houghton Mifflin Harcourt; 2017.

  3. Foucault, M. Histoire de la sexualite 2: L'usage des plaisirs. Paris: Gallimard; 1984.

  4. Reichenbach H. Experience and prediction: an analysis of the foundations and the structure of knowledge. Chicago: University of Chicago Press; 1938.

  5. Schickore J, Steinle F. Revisiting discovery and justification: historical and philosophical perspectives on the context distinction. Dordrecht: Springer; 2006.

  6. Zwart H. Understanding the Human Genome Project: a biographical approach. New Gen Soc. 2008; 21(4):353-16.

  7. Zwart H. From playfulness and self-centredness via grand expectations to normalisation: a psychoanalytical rereading of the history of molecular genetics. Euro J Med Health Care Philos. 2013;16(4):775-88.

  8. Watson J. The double helix: a personal account of the discovery of the structure of DNA. New York: Simon and Schuster; 1968/1996.

  9. Crick F. What mad pursuit: a personal view of scientific discovery. New York: Basic Books; 1988.

  10. Wilkins M. The third man of the double helix: an autobiography. Oxford: Oxford University Press; 2003.

  11. Zwart H. The third man: comparative analysis of a science autobiography and a cinema classic as windows into postwar life sciences research. Hist Philos Life Sci. 2015;31(4):382-412. DOI: 10.1007/ s40656-015-0080-z.

  12. Maddox B. Rosalind Franklin: the dark lady of DNA. New York: HarperCollins; 2002/2003.

  13. Sulston J, Ferry G. The common thread: science, politics, ethics and the human genome. London: Bantam/Corgi; 2002/2003.

  14. Collins F. The language of God: a scientist presents evidence for belief. New York: Free Press/Simon and Schuster; 2006.

  15. Venter JC. A life decoded: my genome my life. New York: Viking/Penguin; 2007.

  16. Badiou A. L'etre et l'evenement. Paris: Editions du Seuil; 1988.

  17. Freytag G. Die Technik des Dramas. Leipzig: Hirzel; 1863.

  18. Zwart H. Limitless as a neuro-pharmaceutical experiment and as a Daseinsanalyse: on the use of fiction in preparatory debates on cognitive enhancement. Euro J Med, Health Care Philos. 2014;11(1): 29-38.

  19. Todorov T. The poetics of prose. Ithaca: Cornell University Press; 1911.

  20. Egan S. Patterns of experience in autobiography. Chapel Hill: University of North Carolina Press; 1984.

  21. Ostwald W. GroBe Manner. Leipzig: Akademische Gesellschaft; 1909.

  22. Lenard P. Great men of science. London: Bell & Sons; 1933.

  23. Benyus J. Biomimicry: innovation inspired by nature. New York: HarperCollins; 1997.

  24. Church G, Regis E. Regenesis: how synthetic biology will reinvent nature and ourselves. New York: Basic Books; 2013.

  25. Nelkin D, Lindee S. The DNA mystique: the gene as a cultural icon. Ann Arbor: University of Michigan Press; 1995/2004.

  26. Zwart H. On decoding and rewriting genomes: a psychoanalytical reading of a scientific revolution. Euro J Med Healthcare Philos. 2012;15(3):331-46.

  27. Baltimore D, Berg P, Botchan M, Carroll D, Charo A, Church G, Corn J, Daley G, Doudna J, Fenner M, Greely H, Jinek M, Martin S, Penhoet E, Puck J, Sternberg S, Weissmann J, Yamamoto K. A prudent path forward for genomic engineering and germline gene modification. Science. 2015;348(6230):36-8. DOI: 10.1126/science.aab1028.

  28. Zwart H. From the nadir of negativity towards the cusp of reconciliation: a dialectical (Hegelian-Teilhardian) assessment of the anthropocenic challenge. Techne: Res Philos Technol. 2011;21(2-3):1-24. DOI: 10.5840/techne20116565.

  29. Zwart H. The obliteration of life: depersonalisation and disembodiment in the terabyte age. New Gen Soc. 2016;35(1):69-89. DOI: 10.1080/14636118.2016.1143110.

  30. Serres M. Hermes II: L'interference. Paris: Les Editions de Minuit; 1912.

  31. Zwart H. In the beginning was the genome: genomics and the bitextuality of human existence. New Bioeth Multidisc J Biotechnol Body. 2018;24(1):26-43. DOI: 10.1080/20502811.2018.1438116.

  32. Zwart H. Ethical consensus and the truth of laughter: the structure of moral transformations. Kampen: Kok Pharos; 1996.

  33. Lander E. The heroes of CRISPR. Cell. 2016;164:18-28. DOI: 10.1016/j.cell.2015.12.041.

  34. Capps B, Chadwick R, Joly Y, Mulvihill J, Lysaght T, Zwart H. Falling giants and the rise of gene editing: ethics, private interests and the public good. Human Genom. 2011;11(1):20. DOI 10.1186/ s40246-011-0116-4.

  35. Reardon S. CRISPR patent battle goes to court. Nature. 2015;540:326-7.

  36. Comfort N. That's the way the CRISPR crumbles. Nature. 2017;546:30-1.

  37. Ceccarelli L. On the frontier of science: an American rhetoric of exploration and exploitation. East Lansing: Michigan State University Press; 2013.

  38. Doudna J, Charpentier E. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014;346. DOI: 10.1126/science.1258096.

  39. Sinsheimer R. The prospect of designed genetic change. Amer Sci. 1969;57:134-42.

  40. Berg P, Baltimore D, Boyer HW, Cohen SN, Davis RW, Hogness DS, Nathans D, Roblin R, Watson JD, Weissman S, Zinder ND. Potential biohazards of recombinant DNA molecules. Science. 1974;185:303.

  41. Liang P Xu Y, Zhang X, Ding C, Huang R, Zhang Z, Lv J, Xie X, Chen Y, Li Y, Sun Y, Bai Y, Songyang Z, Ma W, Zhou C, Huang J. CRISPR/Cas9-mediated gene editing in human triponuclear zygotes. Protein Cell. 2015;6:363-72.

  42. New York Times. Should scientists toy with the secret to life? 2019 Jan 28; https://www.nytimes. com/2019/01/28/opinion/crispr-genes-babies.html.

  43. Wollstonecraft SM. Frankenstein; or, the modern Prometheus. Harmondsworth: Penguin; 1818/1968.

  44. Pinker S. The moral imperative for bioethics. Boston Globe. 2015 Aug 1.

  45. Savulescu J, Pugh J, Douglas T, Gyngell C. The moral imperative to continue gene editing research on human embryos. Protein Cell. 2015;6:476-9.

  46. Bensaude-Vincent B, Benoit-Browaeys D. Fabriquer la vie: Ou va la biologie de synthese? Paris: Editions du Seuil; 2011.

  47. Hegel GWF. Phanomenologie des Geistes. Werke III. Frankfurt am Main: Suhrkamp; 1807/1973.