图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushr.v6.i1.20
18 pages

Ganoderma lucidum (W.Curt.:Fr.) Lloyd and G. applanatum (Pers.) Pat. (Aphyllophoromycetideae) from Slovenian Habitats: Cultivation, Isolation, and Testing of Active Compounds

Bojana Boh
Faculty of Natural Sciences and Engineering, University of Ljubljana, Ljubljana, Slovenia
Marin BEROVIC
Prof.Dr.Bioche.Eng.
Branka Wraber
Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia
Damjan Hodzar
Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Jozica Habijanic
National Institute of Chemistry, Ljubljana, Slovenia ; Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia
Irena Zore
National Institute of Chemistry, Ljubljana, Slovenia

ABSTRACT

Original strains of Ganoderma lucidum (W.Curt.:Fr.) Lloyd and G. applanatum (Pers.) Pat. (synonymous G. lipsiense (Batsch) Atk.) from Slovenian habitats were isolated and used for experimental work. In G. applanatum, research was focused on triterpenoid acids. They were isolated from the fruiting bodies of G. applanatum of Istrian origin (BFWS Gaa2), growing on Populus trees. The highest amount of triterpenoid acids was found in the tubes (6.4 mg/g of air-dried weight), followed by the younger dark context layer of the pileus (2.5 mg/g), the older white context layer (0.6 mg/g), and the upper surface of the fruiting body (0.6 mg/g). G. lucidum strain MZKI G97, isolated from the Slovenian forest, was cultivated by solid state cultivation in a horizontal reactor and by submerged liquid substrate cultivation in a 10-L laboratory stirred tank reactor. Using a 17% (wet weight) of 6-day-old vegetative inoculum, up to 17.0 gL−1 of dry fungal biomass was obtained in a fed batch process. From the mycelium biomass, 1.7 gL−1 of extracellular and 0.45 gL−1 of intracellular polysaccharides were isolated, which were mainly β-D-glucans. Polysaccharide fractions, tested in vitro on the induction of cytokine synthesis in primary cultures of human mononuclear cells from a buffy coat of healthy donors, induced 3.0.630 pg mL−1 of TNF-α and 1.23.2.18 pg mL−1 of IFN-γ.


Articles with similar content:

Submerged and Solid-State Cultivation of Bioactive Extra- and Intracellular Polysaccharides of Medicinal Mushrooms Ganoderma lucidum (W. Curt.: Fr.) P. Karst. and Grifola frondosa (Dicks.: Fr.) S. F. Gray (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 4
Branka Wraber, Jozica Habijanic, Mirjan Svagelj, Bojana Boh, Marin BEROVIC
Studies on Laccase and Biomass Production In Vitro and Culture by a Mexican Wild Strain of Agaricus bisporus (J.Lge) Imbach: a Comparison with Commercial Strains
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
A. Rodriguez-Estrada, Gerardo Mata
Effects of Higher Basidiomycetes Mushrooms on the Hippocampal Slices in Rats
International Journal of Medicinal Mushrooms, Vol.1, 1999, issue 2
Solomon P. Wasser, Mykhaylo Moldavan, Victor M. Storozhuk, Anna A. Grodzinskaya
Polysaccharide Production by Submerged and Solid-State Cultures from Several Medicinal Higher Basidiomycetes
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 1
Sandra Montoya, Oscar Julian Sanchez, Laura Levin
Antitumor Properties of Submerged Cultivated Biomass and Extracts of Medicinal Mushrooms of Genus Hypsizygus Singer (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 1
Anatoliy I. Usov, Elena B. Isakova, Anastasia V. Avtonomova, Larissa M. Krasnopolskaya, Maria I. Leontieva, Igor V. Belitsky, Vladimir M. Bukhman