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国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushr.v7.i4.10
pages 513-524

Anti-β-Glucan Antibody in Bovine Sera

Ken-Ichi Ishibashi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
Chikaku Dogasaki
Faculty of Environmental Health Sciences and School of Veterinary, Azabu University, Sagamihara, Kanagawa, Japan
Tsunenori Iriki
Faculty of Environmental Health Sciences and School of Veterinary, Azabu University, Sagamihara, Kanagawa, Japan
Masuro Motoi
Toei Pharmaceutical Co. Ltd., 2-5-3 Iguchi, Mitaka, Tokyo 181-0011; Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan
Yu-ichiro Kurone
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan
Noriko N. Miura
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
Yoshiyuki Adachi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
Naohito Ohno
Laboratory for Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, Tokyo, Japan

ABSTRACT

We recently detected anti-β-glucan (BG) antibody, which was highly reactive and specific to fungal β-1,3- and β-1,6-BG in sera from human volunteers and DBA/1 and DBA/2 mice. In this study, we examined whether the anti-BG antibody exists in bovine serum and compared the reactivity to various BG fractions from medicinal and edible mushrooms. In experiments using polysaccharide-coated ELISA plates, normal bovine serum showed higher levels of reactivity to the hot water extract of Agaricus brasiliensis, AgHWE. The relative activity is comparable to Candida solubilized cell wall BG, CSBG. On the other hand, the sera weakly reacted to grifolan (GRN) from Grifola frondosa and sonifilan (SPG) from Schizophyllum commune. Furthermore, in a competive ELISA, this antibody cross-reacted to the β-1,6-glucan segment of CSBG and AgHWE. Anti-BG antibody plays a role in the recognition of BG and induces biological activity in collaboration with other recognition molecules, such as the β-glucan receptor. These facts strongly suggest that cows show β-glucan-specific immune response, and β-glucan from mushrooms are recognized and induce immunopharmacological activity in cows.


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