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国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushr.v14.i2.70
pages 189-195

Optimization of Cultivation Conditions of Fermented Shaggy Ink Cap Culinary-Medicinal Mushroom, Coprinus comatus (O.Mull.:Fr.) Pers. (Higher Basidiomycetes) Rich in Vanadium

Chunjing Zhang
Department of Biochemistry and Molecular Biology, Qiqihar Medical University, Qiqihar Heilongjiang, PR China
Xiaodan Qi
Department of Clinical Biochemistry, Qiqihar Medical University, Qiqihar Heilongjiang, PR China
Yan Shi
Department of Biochemistry and Molecular Biology, Qiqihar Medical University, Qiqihar Heilongjiang, PR China
Yan Sun
Department of Clinical Pathogenic Microorganisms, Qiqihar Medical University, Qiqihar Heilongjiang, PR China
Shuyan Li
Department of Biochemistry and Molecular Biology, Qiqihar Medical University, Qiqihar Heilongjiang, PR China
Xiulan Gao
Shandong College of Chinese Medicine, Shandong Yantai, PR China
Haitao Yu
Department of Biochemistry and Molecular Biology, Harbin Medical University, Heilongjiang Harbin; Department of Biology Genetics, Qiqihar Medical University, Qiqihar Heilongjiang, PR China

ABSTRACT

The present paper is mainly aimed at optimization of cultivation conditions of fermented mushrooms of Coprinus comatus rich in vanadium (CCRV). Initial screening of effects of carbon source, temperature, pH, and inoculum size were done by using a one-factor-at-a-time method. The results obtained in that study showed that the optimal medium composition was 30 g glucose/Lin YEPG medium, initial pH 6.0, inoculum volume 10%, and incubation time 120 h. Then the medium was subjected to screening of the most significant parameters using the L9 orthogonal array to solve multivariable equations simultaneously. The results obtained in this study showed that the optimal medium composition was 0.4% V and 30 g glucose/Lin YEPG medium, initial pH 5.0, inoculum volume 15%, and incubation time 120 h. At this medium composition, the mycelial biomass and V content were 7.18 ± 0.24 g/L and 3786.0 ± 17 μg/g, respectively. The anti-diabetic potential of CCRV produced with the optimal level was tested in alloxan-induced diabetes. After the mice were administered (i.g.) with CCRV, the level of blood sugar in the CCRV group was very close to that of the control group. These findings suggested that CCRV produced with the optimal level is useful in the control of diabetes mellitus.


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