图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushrooms.2017024762
pages 1123-1133

Lectin from the Late Oyster Mushroom, Hohenbuehelia serotina (Agaricomycetes), and Its Novel Effect as an Adjuvant of the HBV DNA Vaccine

Yingyin Xu
State Key Laboratory for Agro-Biotechnology, Department of Microbiology and Immunology, China Agricultural University, Beijing, People's Republic of China
Shiwen Chen
State Key Laboratory for Agro-Biotechnology, Department of Microbiology and Immunology, China Agricultural University, Beijing, People's Republic of China
Qinghong Liu
State Key Laboratory for Agro-Biotechnology, Department of Microbiology and Immunology, China Agricultural University, Beijing, People's Republic of China

ABSTRACT

The biological macromolecule Hohenbuehelia serotina lectin (HSL) was first isolated from dried fruiting bodies of the mushroom H. serotina and identified as a heterodimer with 2 subunits of the same molecular weight (15.6 kDa) but different isoelectric points. Lactose and d-galactose inhibited the hemagglutinating activity of HSL, whereas mental ions Mn2+ and Ca2+ could stimulate its hemagglutination. The HSL hemagglutinating activity was stable for 1 hour in NaOH and HCl solutions up to a concentration of 12.5 or 6 mmol/L. In addition, HSL was stable up to 50°C for 30 minutes; its hemagglutinating activity was halved at 60°C and totally inactivated above 90°C. HSL (10 μg) as an immune adjuvant co-inoculated with the proVAX/S2 vaccine enhanced the level of hepatitis B surface antigen in C57BL/6 mice, induced a high level of T-cell proliferation, and induced the expression of IFN of CD4+ cells. We further illustrate that HSL, as an adjuvant upregulating the expression of major histocompatibility complex II, contributed to the maturation of dendritic cells. As the first lectin isolated from H. serotina, HSL is a potential adjuvant to chronic hepatitis B virus DNA vaccines and lays a foundation for the prevention of HBV infection.


Articles with similar content:

Antitumor and Immunomodulatory Effects of Culinary-Medicinal Shiitake Mushroom Lentinus edodes (Berk.) Singer: Analysis of NK Activity, Lymphoproliferative Response, and Antibody Production
International Journal of Medicinal Mushrooms, Vol.6, 2004, issue 4
Ana L. T. Spinardi Barbisan, Fabiani G. Frantz, Lindsey Castoldi, Andrea V. F. Belik, Bianca R. Dias, Augusto Ferreira da Eira, Luciana M. Fontanari, Juliana S. F. Lorenzo, Ramon Kaneno
High Immunomodulatory and Preventive Effects Against Sarcoma 180 in Mice Fed with Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae) Mycelium
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 1
Raffaello Di Bernardi, Rosalia Rubel, Jose Hermenio Cavalcante Lima Filho, Carlos Ricardo Soccol, Luiz Claudio Fernandes, Andrea N. Moreno, Fan Leifa, Herta Stutz Dalla Santa, Bonald Cavalcante Figueiredo
T-Cell Control of Immunity to the Asexual Blood Stages of the Malaria Parasite
Critical Reviews™ in Immunology, Vol.14, 1994, issue 2
Klavs Berzins, Marita Troye-Blomberg, Peter Perlmann
Elimination of Virus-Specific Cytotoxic T Cells in the Liver
Critical Reviews™ in Immunology, Vol.22, 2002, issue 1
Gunther Dennert
Antitumor and Immunomodulating Activities of Exopolysaccharide Produced by Big Cup Culinary- Medicinal Mushroom Clitocybe maxima (Higher Basidiomycetes) in Liquid Submerged Culture
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 9
Peter Chi Keung Cheung, Yu-Kuei Chen, Jinn-Chyi Wang, Shu-Hui Hu, Raw-Pou Hung, Sue-Joan Chang