图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushrooms.2017024404
pages 915-924

Anti-Inflammatory Activity and Bioactive Constituents of Cultivated Fruiting Bodies of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus

Chih-Chuang Liaw
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan
Shu-Jing Wu
Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan
Ching-Fu Chen
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan
Ming-Nan Lai
Kang Jian Biotech Co., Ltd., Nantou, Taiwan
Lean Teik Ng
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan

ABSTRACT

Xylaria nigripes, also known as Wu Ling Shen, is popular for treating insomnia and trauma in traditional Chinese medicine. This study aimed to examine the anti-inflammatory activity and bioactive constituents of cultivated X. nigripes fruiting bodies in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Results showed that among the different extracts, the hexane fraction exhibited the best protection against cell toxicity induced by 1 μg/mL LPS and the strongest inhibitory effect on nitric oxide (NO) production. This fraction led to the isolation of 2 bioactive compounds (namely, XN-CP1 and XN-CP2), which were confirmed to be ergostarien-3β-ol and ergosterol peroxide, respectively. Although both XN-CP1 and XN-CP2 showed good inhibitory effects on NO, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and prostaglandin E2 production in LPS-stimulated macrophages, XN-CP2 was shown to have a stronger anti-inflammatory activity; this was further supported by its strong suppressive effects on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression and nuclear factor (NF)-κB activation. These results conclude that ergosterol peroxide (XN-CP2) could be the main bioactive compound contributing to the potent anti-inflammatory activity of X. nigripes, and its mechanism of action is mediated through inhibition of iNOS and COX-2 expression via the NF-κB signaling pathway.


Articles with similar content:

Anti-Inflammatory Activity of Biomass Extracts of the Bay Mushroom, Imleria badia (Agaricomycetes), in RAW 264.7 Cells
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 9
Joanna Gdula Argasinska, Agata Grzywacz, Wlodzimierz Opoka, Katarzyna Kala, Bozena Muszynska
Oleanolic acid induces Apoptosis by modulating p53, Bax, Bcl-2 and caspase-3 gene expression and regulates the activation of transcription factors and cytokine profile in B16F
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 1
P. Pratheeshkumar, Girija Kuttan
Dichlormethane Extract of the Jelly Ear Mushroom Auricularia auricula-judae (Higher Basidiomycetes) Inhibits Tumor Cell Growth In Vitro
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 1
Md.Ahsanur Reza, Md. Akil Hossain, Seung-Chun Park, Woo-Sik Jo, Seung-Jin Lee, Joo-Won Suh, Sileshi Belew Yohannes, Dereje Damte, Man-hee Rhee
Influence of Navisporus floccosus (Higher Basidiomycetes) n-Hexane Extract on Arachidonate Metabolism in Vitro
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 5
Sabine Ortmann, Vivian Leliebre-Lara, Clara Nogueiras Lima, Ute Widowitz, Eva-Maria Pferschy-Wenzig, Rudolf Bauer
The Anti-Inflammatory Effects of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Higher Basidiomycetes) in a Coculture System of 3T3-L1 Adipocytes and RAW264 Macrophages
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 7
Noriyasu Hirasawa, Kenji Ouchi, Koichiro Mori