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国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushrooms.v17.i9.20
pages 819-828

Antiviral Activity of a Cloned Peptide RC28 Isolated from the Higher Basidiomycetes Mushroom Rozites caperata in a Mouse Model of HSV-1 Keratitis

Naihong Yan
Department of Ophthalmology and Ophthalmic Laboratories, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China
Fen He
Department of Ophthalmology and Ophthalmic Laboratories, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China
Frank F. Piraino
Department. of Visual Sciences, University of Wisconsin Medical School, Madison 517 Rupert Rd., Waunakee, WI. 53597, USA
Haotian Xiang
Department of Ophthalmology and Ophthalmic Laboratories, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China
Jun Chen
Department of Ophthalmology and Ophthalmic Laboratories, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China
Yun Wang
Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, Shenzhen, China
Xuyang Liu
Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China

ABSTRACT

An Escherichia coli−expressed peptide with a molecular weight of 28.26, derived from the complementary DNA of antiviral protein RC28 isolated from the mushroom Rozites caperata (=Cortinarius caperatus), demonstrated potent antiviral activity against herpes simplex virus-1 in Vero cells and in a herpes simplex virus-1 mouse keratitis model. Plaque assays in Vero cells showed that the peptide reduced viral yields by at least 1.2 logs; in the animal model the cloned peptide delayed the occurrence of stromal keratitis and alleviated the severity of the disease. We believe this is the first report of a cloned mushroom peptide with antiviral activity for the prevention and treatment of a viral disease.


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