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国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushrooms.v18.i4.10
pages 279-289

A Search for the Optimum Selenium Source to Obtain Mushroom-Derived Chemopreventive Preparations

Milena Savic
Department of Food and Industrial Microbiology, Faculty of Agriculture, University of Belgrade, Belgrade, Serbia
Marzenna Klimaszewska
Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Warsaw, Poland
Magdalena Bamburowicz-Klimkowska
Department of Toxicology, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland
Piotr Suchocki
Department of Drug Analysis, Medical University of Warsaw, Warsaw, Poland; National Medicines Institute, Department of Pharmaceutical Chemistry, Warsaw, Poland
Miomir Niksic
lnstitute for Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Belgrade, Serbia
Miroslaw Szutowski
Department of Toxicology, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland
Piotr Wroczynski
Department of Drug Analysis, Medical University of Warsaw, Warsaw, Poland
Jadwiga Turlo
Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Warsaw, Poland

ABSTRACT

The objective of this research was to test whether selenium−yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides. Culture media were enriched in selenium to a concentration of 20 µg/mL. Selenium was added to the medium either in the form of sodium selenite or in form of Se-yeast (Sel-Plex; Alltech Inc., Lexington, KY). The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides were determined using atomic absorption spectroscopy. We found that selenium accumulated more efficiently in cultures enriched with Se-yeast. A higher concentration of selenium was also found in the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media. With the use of the needle trap gas chromatography−mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.