图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushr.v15.i2.10
pages 115-126

Analysis of the Titer and Reactivity of Antibody/ies Against Fungal Cell Wall β-Glucans in Human Sera

Ken-Ichi Ishibashi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
Mayu Morita
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan
Masuro Motoi
Toei Pharmaceutical Co. Ltd., 2-5-3 Iguchi, Mitaka, Tokyo 181-0011; Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan
Ying Liu
Research Center for Food Safety, The University of Tokyo; Mibyou Medical Research Center, Institute of Preventive Medicine, Setagaya-ku, Tokyo, Japan
Noriko N. Miura
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
Yoshiyuki Adachi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
Naohito Ohno
Laboratory for Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, Tokyo, Japan

ABSTRACT

The aim of this work was to characterize an antibody response to β-glucan (BG), a major component of the fungal cell wall, at each isotype in human sera. The titer and reactivity of the anti-human BG antibody were examined using enzyme-linked immunosorbant assay plates coated with Candida soluble cell wall β-glucan as a standard antigen. The antibody was detected using anti-human immunoglobulin (Ig) G, IgM, and IgA. Its major class was IgG in all subjects. The antibody titer varied significantly. The anti-BG antibody showed greater reactivity to β-glucans derived from pathogenic fungi than monoglycosyl-branched β-glucans derived from mycelia culture medium. In addition, it was suggested that the anti-BG IgM antibody was bound relatively strongly to the β1,3-glucan backbone and the anti-BG IgG antibody to β1,6-glucan. The anti-BG antibody plays a variety of roles, according to class, in the host's response to fungi. We propose a new index of human response to β-glucan that effects the understanding of the response to β-glucan in humans.


Articles with similar content:

Anti-β-Glucan Antibody in Bovine Sera
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 4
Yoshiyuki Adachi, Noriko N. Miura, Tsunenori Iriki, Yu-ichiro Kurone, Naohito Ohno, Ken-Ichi Ishibashi, Chikaku Dogasaki, Masuro Motoi
Tumor Cell Recognition by Lymphocytes: Is the MHC Always Essential?
Critical Reviews™ in Immunology, Vol.18, 1998, issue 1-2
Beverly Z. Packard , Akira Komoriya
Immunomodulatory Effects of Extracellular β-Glucan Isolated from the King Oyster Mushroom Pleurotus eryngii (Agaricomycetes) and Its Sulfated Form on Signaling Molecules Involved in Innate Immunity
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 6
Bharat Bhusan Patnaik, Hyoun-Su Lee, Yong Hyun Kim, Hyun-Jung Kwon, Kook-Il Han, Eui-Gil Jung, Wan Jong Kim, Man-Deuk Han
β-Glucan of Ganoderma lucidum (W.Curt.:Fr.) Lloyd (Aphyllophoromycetideae) Cooperatively Induces Tumor Necrosis Factor-α and Interleukin-6 with Lipopolysaccharide by Binding to Dectin-1
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 1&2
Mi Jeong Kim, Ha Won Kim, Se Young Choung, Dong Hee Lee, Byong Kak Kim, Su Kyoung Sung
Disruption of Actin Cytoskeleton Enhanced Cytokine Synthesis of Splenocytes Stimulated with Beta-Glucan from the Cauliflower Medicinal Mushroom, Sparassis crispa Wulf.:Fr. (Higher Basidiomycetes) in Vitro
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 3
Yoshiyuki Adachi, Noriko N. Miura, Asami Shibata, Naohito Ohno, Ken-Ichi Ishibashi, Toshie H. Hida