图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushr.v13.i1.70
pages 51-60

Production of Bioactive Exopolysaccharides from Bitter Medicinal Mushroom, Antrodia camphorata (M. Zang et C.H. Su) Sh.H. Wu et al. (Aphyllophoromycetideae) in Submerged Cultivation

Ming-Yeou Lung
Department of Chemical and Materials Engineering, Minghsin University of Science and Technology, Hsin Fong, Hsin Chu, Taiwan 304, R.O.C.
Ching-Yi Lee
Department of Chemical and Materials Engineering, Minghsin University of Science and Technology, Xinfeng, Hsinchu, Taiwan, R.O.C.
Wen-Xian Chen
Department of Chemical and Materials Engineering, Minghsin University of Science and Technology, Xinfeng, Hsinchu, Taiwan, R.O.C.
Ernest Huang
Department of Chemical and Materials Engineering, Minghsin University of Science and Technology, Xinfeng, Hsinchu, Taiwan, R.O.C.

ABSTRACT

This study examines the effects of various fructose concentrations in media on the production and quality of bioactive exopolysaccharides (EPS) from Bitter medicinal mushroom, Antrodia camphorate in submerged cultures. The fructose in media of submerged cultures of A. camphorate significantly affected the production, average molecular weight (Mn), and antioxidant activity of exopolysaccharides. The specific growth rate decreased monotonically from 0.33 to 0.25 1/day as the fructose concentration increased from 10 to 60 g/L; however, maximum production and productivity for EPS increased from 75.23 to 164.87 mg/L and 6.27 to 9.70 mg/L/day, respectively. In addition, the fed-batch culture used in this study significantly improved the production of EPS (2.43-fold enhancement, from 75.23 to 182.99 mg/L), number average molecular weights of EPS (1.47-fold enhancement, from 5.44 × 105 to 7.98 × 105 Da), protein/exopolysaccharide ratios (1.63-fold enhancement, from 16% to 26%), and antioxidant activity of EPS (1.32-fold enhancement, from 60% to 79%), as compared with corresponding properties of batch fermentation at 10 g/L fructose in an air-lift bioreactor. The antioxidant activity of EPS was highly correlated with number average molecular weights (R2 = 0.90) and protein/exopolysaccharide ratios (R2 = 0.96). The positive results of this research have successfully verified the promotion efficiency on the production and quality of EPS from the medicinal mushroom A. camphorate.


Articles with similar content:

Medium Selection for Exopolysaccharide and Biomass Production in Submerged Cultures of Culinary-Medicinal Mushrooms from Turkey
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 1
Mustafa Yamac, Meltem Kizilcik
Extraction Optimization, Composition Analysis, and Antioxidation Evaluation of Polysaccharides from White Jelly Mushroom, Tremella fuciformis (Tremellomycetes)
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 12
Yu Zou, Xiyan Hou
Effect of Carbon Source on Properties and Bioactivities of Exopolysaccharide Produced by Trametes ochracea (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.22, 2020, issue 3
Yichang Chen, Xuewei Jia, Chunping Xu, Jiafeng Bai, Shaohua Liu, Zhengxing Ning
The Influence of Cultivation Conditions on Mycelial Growth and Exopolysaccharide Production of Culinary-Medicinal Mushroom Pleurotus citrinopileatus Singer (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 3
Chiu-Yeh Wu
Chemical Characterization and Structure of Exopolysaccharides from Submerged Culture of New Medicinal Mushroom from China, Phellinus mori (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 1
Feng Peng, Bao-Kai Cui, Chunlei Cao