图书馆订阅: Guest
神经生物学评论综述™

每年出版 3 

ISSN 打印: 0892-0915

ISSN 在线: 2375-0014

SJR: 0.121

Spatio-Temporal Regulation of Neurotransmitter Release by PKC; Studies in Adrenal Chromaffin Cells

卷 16, 册 1&2, 2004, pp. 173-179
DOI: 10.1615/CritRevNeurobiol.v16.i12.180
Get accessGet access

摘要

Activation of protein kinase C (PKC) seems to promote vesicle recruitment to the release-ready state prior to Ca2+-triggered fusion in chromaffin cells. To understand spatio-temporal regulation of vesicle recruitment by PKC, we studied the effects of a phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), on the vesicle movements in living chromaffin cells by imaging with a fluorescence microscope-cooled CCD system. About 60~80% of the chromaffin vesicles showed a rapid movement, about 20% showed a moderate movement, and the rest showed slow/no movement in resting and post-stimulation. The vesicles with slow/no movement increased to 40% upon a depolarizing stimulation, and TPA increased this population to about 70%. TPA treatment, in addition, increased the number of visible chromaffin vesicles beneath the plasma membrane, suggesting that the potentiation of vesicle recruitment by PKC involves a substantial increase in the subplasmalemmal distribution of vesicles.

对本文的引用
  1. Hussain Rifat J., Stumpo Deborah J., Blackshear Perry J., Lenox Robert H., Abel Ted, McNamara Robert K., Myristoylated alanine rich C kinase substrate (MARCKS) heterozygous mutant mice exhibit deficits in hippocampal mossy fiber-CA3 long-term potentiation, Hippocampus, 16, 5, 2006. Crossref

  2. Rosmaninho-Salgado Joana, Araújo Inês M., Álvaro Ana Rita, Duarte Emília P., Cavadas Cláudia, Intracellular signaling mechanisms mediating catecholamine release upon activation of NPY Y1receptors in mouse chromaffin cells, Journal of Neurochemistry, 103, 3, 2007. Crossref

  3. Rosmaninho-Salgado Joana, Araújo Inês M., Álvaro Ana Rita, Mendes Alexandrina F., Ferreira Lígia, Grouzmann Eric, Mota Alfredo, Duarte Emília. P., Cavadas Cláudia, Regulation of catecholamine release and tyrosine hydroxylase in human adrenal chromaffin cells by interleukin-1β: role of neuropeptide Y and nitric oxide, Journal of Neurochemistry, 109, 3, 2009. Crossref

  4. Cortez Vera, Santana Magda, Marques Ana Patrícia, Mota Alfredo, Rosmaninho-Salgado Joana, Cavadas Cláudia, Regulation of catecholamine release in human adrenal chromaffin cells by β-adrenoceptors, Neurochemistry International, 60, 4, 2012. Crossref

  5. Watanabe Michitoshi, Nomura Kazushige, Ohyama Akihiro, Ishikawa Ryoki, Komiya Yoshiaki, Hosaka Kohei, Yamauchi Emiko, Taniguchi Hisaaki, Sasakawa Nobuyuki, Kumakura Konosuke, Ushiki Tatsuo, Sato Osamu, Ikebe Mitsuo, Igarashi Michihiro, Myosin-Va Regulates Exocytosis through the Submicromolar Ca2+-dependent Binding of Syntaxin-1A, Molecular Biology of the Cell, 16, 10, 2005. Crossref

Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集 订购及政策 Begell House 联系我们 Language English 中文 Русский Português German French Spain