图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
国际藻类期刊
SJR: 0.216 SNIP: 0.322 CiteScore™: 0.4

ISSN 打印: 1521-9429
ISSN 在线: 1940-4328

国际藻类期刊

DOI: 10.1615/InterJAlgae.v19.i4.80
pages 385-396

Rapid Method for Simultaneous Discrimination of Microalgae and Determination on Biochemical Composition based on Vibrational Spectroscopy

P. Sureshkumar
Algae Biomass Research Laboratory, Department of Biosciences & Technology, Karunya University, Karunya Nagar, Coimbatore 641114, India
J. Thomas
Algae Biomass Research Lab., Department of Biotechnology, School of Agriculture and Biosciences, Karunya Institute of Technology & Sciences, Coimbatore 641114, Tamil Nadu, India
J. Sivasubramanian
Department of Physics, Madura College, Madurai 625011 Tamil Nadu, India

ABSTRACT

Fourier-transform infrared (FT-IR) spectroscopy is a high-resolution spectroscopic method used in whole-organism molecular fingerprinting. This analysis helps for the characteristic determination of macromolecular spectrum in intact cells. Spectral analysis can discriminate, classify, and identify the microorganisms as composition of macromolecules is different between strains of the same species. In the present investigation, four different microalgae were studied for their variations in spectral data, which showed striking differences on their major macromolecules. Scenedesmus obliquus (Turp.) Kütz. have intense peaks in the lipids and protein spectral regions when compared to the other species. Chlorella vulgaris Beij., Ch. pyrenedosa Chick and Euglena gracilis Klebs possessed moderate and less intense peaks in sub sequential order in all the five spectral regions. Using FT-IR spectra, hierarchical clustering analyses resulted in a dendrogram with clear discrimination between species according to their phenotype variations. To support this, phylogenetic analysis using 16S rDNA microbial barcode sequences of these microalgal strains was evaluated and the resulting phylogram showed the same cluster pattern as revealed by FT-IR based dendrogram. Hence, this study concludes that the combination of phylogenetic analysis and the FTIR spectra provides an effective way of distinguishing the species as well as the advantage of simultaneous determination of biochemical composition of the species.


Articles with similar content:

Molecular Identification of Some Medicinally Important Aphyllophorales Mushrooms Based on ITS rDNA Sequences and RAPD Data
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 4
S. K. Singh, Shwet Kamal, R. D. Rai
Medicinal Species from Genera Inonotus and Phellinus (Aphyllophoromycetideae): Cultural-Morphological Peculiarities, Growth Characteristics, and Qualitative Enzymatic Activity Tests
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 3
Solomon P. Wasser, Ivan V. Zmitrovich, Daniel Tura, Eviatar D. Nevo
Genetic Variability of the Medicinal Tinder Bracket Polypore, Fomes fomentarius (Agaricomycetes), from the Asian Part of Russia
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 6
Susanna M. Badalyan, Victor A. Mukhin, Elena V. Zhuykova
Algicidic Properties of Polyphenols Depending on Their Molecular Structure
Hydrobiological Journal, Vol.40, 2004, issue 6
A. I. Dakevich
Fluorescence Spectroscopy Application in the Express-evaluation of the Gastrointestinal Microflora
Critical Reviews™ in Biomedical Engineering, Vol.28, 2000, issue 5&6
V. V. Chernyi, S. Yu. Koz'ma, I. M. Taubinsky, M. T. Alexandrov