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生物医学的图像处理,计算和显示


ISSN 在线: 2162-3511

Archives: Volume 1, 2012 to Volume 2, 2013

生物医学的图像处理,计算和显示

DOI: 10.1615/VisualizImageProcComputatBiomed.2013005967

A Five-Axis Arm-Manipulator Laser System and an Algorithm for Digital Processing of Output Data for Recording and Morpho-Topological Identification of Cell and Tissue Structures in Histomorphogenesis

Alexander V. Notchenko
Neuronal Brain Structure Laboratory, Scientific Neurology Center, Russian Academy of Medical Sciences; Bauman Moscow State Technical University
O. V. Gradov
Neuronal Brain Structure Laboratory, Scientific Neurology Center, Russian Academy of Medical Sciences; Institute of Chemical Physics, Russian Academy of Sciences

ABSTRACT

An automated system for morpho-topological determination of cell division phases and structural differentiation of tissues during morphogenesis was implemented on the basis of topological properties of cell cultures, analyzed within the naïve theory of sets. A simple robotic hardware and software system based on a Zeiss microscope with a modified stage and a Velleman manipulator KSR-1 allows controlling the laser module position, carrying out the angular irradiation of samples both in transmission and in darkfield or luminescent modes and the subsequent mathematical data processing. This low-budget system can be easily assembled and programmed in any cytomorphological or histomorphological laboratory using available materials at hand. The code for data processing in MATLAB is given at the end of the paper.


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