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生物医学的图像处理,计算和显示


ISSN 在线: 2162-3511

Archives: Volume 1, 2012 to Volume 2, 2013

生物医学的图像处理,计算和显示

DOI: 10.1615/VisualizImageProcComputatBiomed.2013005968

Elementary Morphometric Labs-on-a-Chip Based on Hemocytometric Chambers with Radiofrequency Culture Identification and Relay of Spectrozonal Histochemical Monitoring

Alexander V. Notchenko
Neuronal Brain Structure Laboratory, Scientific Neurology Center, Russian Academy of Medical Sciences; Bauman Moscow State Technical University
O. V. Gradov
Neuronal Brain Structure Laboratory, Scientific Neurology Center, Russian Academy of Medical Sciences; Institute of Chemical Physics, Russian Academy of Sciences

ABSTRACT

An accessible design of autonomous labs-on-the-chip, which do not require a special reader but use grid hemocytometric counting chambers for sectioning the detected cells, isolated by a special device for cell sedimentation, is proposed. A system for automated radiofrequency (RF) identification of chambers in long-term storage conditions, which provides new morphometric data at various stages of cultivation or biomonitoring, is proposed. A new diffraction method for calculating and fingerprinting cellular structures in varying environmental conditions is described. Experimental data on the pilot testing of Fuchs-Rosenthal, Buerker, Neubauer, Makler, and Thoma chambers for the described technique are presented. The applicability of these devices for coherent-densitometric indication either of concentration dynamics in cell culture or of suspension and morphogenetic processes in them for laminar conditions is shown. A self-learning program for cellular pattern recognition and precision cytophotometry based on a lab-on-a-chip with micrometric object calibration relative to the counting net is demonstrated. A hybrid in vitro/in silico method of morphogenesis monitoring in cell cultures is introduced.


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