RT Journal Article ID 002de0371cd2e463 A1 Rohini, Baradhan A1 Akther, Tahira A1 Waseem, Mohammad A1 Khan, Jasim A1 Kashif, Mohammad A1 Hemalatha, Srinivasan T1 AgNPs from Nigella sativa Control Breast Cancer: An In Vitro Study JF Journal of Environmental Pathology, Toxicology and Oncology JO JEP(T) YR 2019 FD 2019-05-09 VO 38 IS 2 SP 185 OP 194 K1 AgNPs K1 breast cancer K1 apoptosis K1 mitochondria AB In our current study, we synthesized silver nanoparticles (AgNPs) from an aqueous seed extract of Nigella sativa. The seed extract contains phytochemical compounds including phenols, terpenoids, and flavonoids that may act as reducing agents and are able to convert metal ions to metal nanoparticles. The formation of synthesized AgNPs was characterized using UV-visible spectroscopy, Fourier transform infra-red spectroscopy (FT-IR), scanning electron microscopy (SEM) and energy dispersive analysis of X-rays (EDX). The efficacy of N-AgNPs against human breast cancer (MCF-7) cells was tested. The synthesized AgNPs displayed dose-dependent cytotoxicity (1−200 µg/mL) against MCF-7 cells. Morphological alterations of the cells also appeared as bright field images. Treatment of synthesized AgNPs altered the expression of Bax and Bcl-2 (apoptotic proteins) and COX-2 (inflammatory marker) in MCF-7 cells. To our knowledge, this is the first report demonstrating that N-AgNPs from Nigella sativa can induce apoptosis in MCF-7 cells. PB Begell House LK https://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,2a1f3ff2521fee54,002de0371cd2e463.html