RT Journal Article
ID 2d9d833a03d8c13c
A1 Yurovsky, Vladimir V.
T1 The Repertoire of T-Cell Receptors in Systemic Sclerosis
JF Critical Reviews™ in Immunology
JO CRI
YR 1995
FD 1995-06-30
VO 15
IS 2
SP 155
OP 165
K1 T cell subsets
K1 bronchoalveolar lavage
K1 T cell receptor
K1 diversity
K1 polymerase chain reaction.
AB Certain T cell subsets are increased in systemic sclerosis patients, particularly Vδ1+ γδ T cells in the blood and lungs and CD8+ αβ T cells in the lungs. The repertoires of T cell antigen receptor (TCR) Vδl, Vα, and Vβ gene families were examined by two methods of analysis. First, the relative abundance of Vα and Vβ gene transcripts was determined in the blood and bronchoalveolar fluid of the patients. Second, the diversity of the junctional regions in TCR Vδl transcripts and in different Vα and Vβ gene families was analyzed. Limited Vδ-Cδ junctional region lengths were observed in the patients compared with controls. This was confirmed by sequence analysis of Vδ1-Cδ junctional regions after subcloning amplified products in a bacterial vector. Evidence for selection of the Vδl+ T cells in the tissues of patients came from the findings that the same Vδ1-Cδ junctional sequences persisted in an individual patient over time and that identical junctional sequences were isolated from multiple sites. A restricted diversity of the junctional region lengths was also detected in a number of Vα and Vβ gene families, particularly within bronchoalveolar CD8+ T cell subset. These data suggest that the oligoclonal expansion of the corresponding αβ and γδ T cells is antigen-driven and may be important in the pathogenesis of systemic sclerosis.
PB Begell House
LK https://www.dl.begellhouse.com/journals/2ff21abf44b19838,38f196a61569eb05,2d9d833a03d8c13c.html