RT Journal Article ID 2e801e9d0be4b07d A1 Hammer, Malte U. A1 Forbrig, E. A1 Kupsch, S. A1 Weltmann, Klaus-Dieter A1 Reuter, Stephan T1 IInfluence of Plasma Treatment on the Structure and Function of Lipids JF Plasma Medicine JO PMED YR 2013 FD 2014-10-01 VO 3 IS 1-2 SP 97 OP 114 K1 plasma medicine K1 plasma treatment K1 nonthermal plasma K1 membrane K1 bilayer K1 lipids K1 lipid oxidation K1 plasma poration K1 lesions K1 self-mediated in- and efflux AB The membrane of both pro- and eukaryotic cells is the cell's interface with the environment. It is the first interaction site of any substance that is externally applied, including reactive species in the liquid cell environment created by plasma medical treatments. Therefore, the liquid surrounding the cell is, due to its influence on the chemical paths, an important mediator for plasma-borne reactive species, and the cellular membrane is their primary target structure. A cellular membrane consists, according to the Singer−Nicolson model, of a lipid bilayer with embedded proteins. Here, we describe experiments of plasma treatments of lipids and liposomal model membranes. The investigations show membrane activity of plasma-borne reactive species against lipids and lipid structures. The methods applied are Raman microscopy and chromophore-based light spectroscopy. Results of dynamic light scattering (DLS) and fluorophore-based assays show that, during the applied plasma treatment, neither macroscopic collapse of the lipid superstructure nor liposome fusion was observed. Raman spectroscopy reveals increased fluidity of lipid layers due to plasma treatment. The results are discussed based on our observations and published results. We propose a detailed molecular mechanism for the formation of lesions that allow a "self-mediated in- and efflux" of plasma-borne reactive species and cell signaling molecules. Resulting consequences for cellular membranes and the cell as a whole are discussed. PB Begell House LK https://www.dl.begellhouse.com/journals/5a5b4a3d419387fb,4eec539e3367d43b,2e801e9d0be4b07d.html