RT Journal Article ID 30181a9427a1f563 A1 Wang, Mingxing A1 Zhang, Yanqiu A1 Xiao, Xulang A1 Xu, Duoduo A1 Gao, Yang A1 Gao, Qipin T1 A Polysaccharide Isolated from Mycelia of the Lion’s Mane Medicinal Mushroom Hericium erinaceus (Agaricomycetes) Induced Apoptosis in Precancerous Human Gastric Cells JF International Journal of Medicinal Mushrooms JO IJM YR 2017 FD 2018-02-05 VO 19 IS 12 SP 1053 OP 1060 K1 apoptosis K1 Hericium erinaceus K1 medicinal mushrooms K1 polysaccharide K1 precancerous human gastric cells AB Hericium erinaceus is typically used in traditional Chinese medicine for mucosal protection, healing of gastric ulcers, and treatment of gastritis. We purified from the cultured mycelia of H. erinaceus a polysaccharide with anti–gastric ulcer and antigastritis activity, but its effects on gastric malignancy have not been elucidated. We examined the differential effects of this purified polysaccharide, named EP-1, on the human gastric (GES-1) cell line and a precancerous cell line (MC) that was transformed from GES-1 using N-methyl-N'-nitro-N-nitrosoguanidine. We observed that the polysaccharide potently induced cell apoptosis and cell cycle arrest at the G0/G1 phase in the MC cell line but did not have any effect on the GES-1 cell line at the same doses. Further mechanistic studies revealed that the polysaccharide exerted its activity through an apoptosis-associated pathway by modulating the expression of Bax, Bcl-2, and caspase-3. Differential effects of the polysaccharide on the GES-1 and MC cell lines indicate that the polysaccharide was effective in preventing gastric cancer progression. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2dab30ba797125f4,30181a9427a1f563.html