RT Journal Article ID 33d5173438d2ff40 A1 Zhu, Hu A1 Liu, Wei A1 Tian, Baozhen A1 Liu, Huijun A1 Ning, Shoujiao T1 Inhibition of Quorum Sensing in the Opportunistic Pathogenic Bacterium Chromobacterium violaceum by an Extract from Fruiting Bodies of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.) P. Karst. (Higher Basidiomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2011 FD 2011-12-15 VO 13 IS 6 SP 559 OP 564 K1 medicinal mushrooms K1 Ganoderma lucidum methanol extract K1 Chromobacterium violaceum CV026 K1 N-hexanoyl homoserine lactone K1 anti-quorum sensing K1 violacein AB Extracts of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum, inhibited quorum sensing in Chromobacterium violaceum CV026. G. lucidum fruiting bodies were milled and extracted with ethyl acetate. The crude extract was dissolved in an appropriate concentration of methanol, sterilized by filtration through a 0.22-μm membrane filter, and added to Ch. Violaceum CV026 cultures, which were used as an indicator to monitor quorum sensing inhibition. Inhibitory activity was measured by quantifying violacein production using a microplate reader. Methanol-soluble compounds extracted from G. lucidum significantly inhibited quorum sensing-controlled behavior in Ch. Violaceum in a concentration-dependent manner. The results suggest that compounds in G. lucidum might be useful to control and handle detrimental infections caused by human, animal, and plant pathogens. Further studies are in progress in our lab to isolate the specific compounds from G. lucidum extract, evaluate them as quorum sensing inhibitors, and analyze their mechanism of action. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7b5e519241ca8193,33d5173438d2ff40.html