RT Journal Article ID 374e55ec3fe2edf9 A1 Zhang, Hua A1 Zhao, Youchun A1 Zhang, Hanxing A1 Feng, Yongjun A1 Zhang, Changkai T1 Quantitative Method Measurement of Flammulin Concentration with Immunoassay JF International Journal of Medicinal Mushrooms JO IJM YR 2002 FD 2002-09-01 VO 4 IS 3 OP 4 AB Flammulin purified from the aqueous extract of basidiomes of edible and medicinal mushroom Flammulina velutipes was completely emulsified with Freund adjuvant, then injected into New Zealand white rabbits. After several immunity enhancements, these animals were bled and serum was separated. Double diffusion showed that the antiserum could react with flammulin and form the precipitate cycle in agarose gel with valence of 1:16. According to the result of simple agar diffusion of flammulin with serum on agarose gel plate, a group of standard curves, fitting the reaction results of flammulin at different concentrations, were established, which can be used in the quantitative measurement of flammulin concentration. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7a31e55f2119a143,374e55ec3fe2edf9.html