RT Journal Article
ID 47de69fc15240003
A1 Sysolyatina, Elena V.
A1 Mukhachev, Andrey
A1 Yurova, Maria
A1 Grushin, Mikhail
A1 Karal'nik, Vladimir
A1 Petryakov, Alexander
A1 Trushkin, Nikolai
A1 Danilova, Maria
A1 Naroditsky, Boris S.
A1 Gintsburg, Alexander L.
A1 Akishev, Yuri
A1 Ermolaeva, Svetlana A.
T1 Experimental Evidences on Synergy of Gas Discharge Agents in Bactericidal Activity of Nonthermal Plasma
JF Plasma Medicine
JO PMED
YR 2013
FD 2014-10-01
VO 3
IS 1-2
SP 137
OP 152
K1 nonthermal plasma
K1 sterilization
K1 Staphylococcus aureus
K1 Pseudomonas aeruginosa
AB Nonthermal plasmas (NTPs) represent a new class of sterilizing agents. A full set of NTP bioactive factors includes electric components (E, charged particles and electric field), neutral active particles (R), and UV. A specific construction of the direct current (DC) corona source was used that allowed dissection of NTP bioactive factors and quantitative evaluation of their individual and combined action on bacterial pathogens Pseudomonas aeruginosa and Staphylococcus aureus. 10 and 120 s were required for the positive and negative coronas, respectively, to kill 105 colony-forming units (CFU) P. aeruginosa. 10 s was required for both positive and negative coronas to kill 105 CFU S. aureus. For the positive corona, the bactericidal activity of components decreased as R+UV>E+UV>>UV and E+UV>R+UV>>UV for P. aeruginosa and S. aureus, respectively. For the negative corona, the bactericidal activity decreased as R+UV>>E+UV=UV=0 and R+UV>>E+UV>UV~0 for P. aeruginosa and S. aureus, respectively. Despite low, if any, activity of electric components in the negative corona, the whole plasma effect was much higher than the effect of neutral particles and UV alone. The obtained results demonstrated that different combinations of bioactive plasma components exerted diverse species-specific bactericidal effects. It is synergy among plasma bioactive factors that supplies high bactericidal activity of NTP.
PB Begell House
LK https://www.dl.begellhouse.com/journals/5a5b4a3d419387fb,4eec539e3367d43b,47de69fc15240003.html