RT Journal Article ID 486c113c0efd1d6a A1 Jia, Xuewei A1 Qu, Lili A1 Panpan, Ran A1 Liu, Shuai A1 Wu, Yan A1 Xu, Chunping T1 Characterization and Antioxidant Activity of an Exopolysaccharide Produced by Rigidoporus microporus (Agaricomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2018 FD 2018-05-16 VO 20 IS 4 SP 311 OP 320 K1 antioxidant K1 exopolysaccharide K1 medicinal mushrooms K1 optimization K1 Rigidoporus microporus AB In this investigation we developed an optimal fermentation medium to produce an exopolysaccharide (EPS) with antioxidant activity from Rigidoporus microporus. Lactose and tryptone were chosen as the optimal carbon and nitrogen sources, respectively, for EPS production, with a 6-day cultivation cycle. After removing proteins through the use of the Sevag method, one EPS fraction was purified from the culture filtrates by gel filtration chromatography on a Sepharose CL-6B column. The preliminary chemical structure of the EPS fraction was determined by Fourier transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance. The results indicated that the main structure of the EPS consisted of β-glucopyranose and mannopyranose. Furthermore, conformational parameters such as molecular weight (Mw), intrinsic viscosity ([η]), mean-square radii of gyration (Rg), and hydrodynamic radius (Rh) of the EPS were characterized using a size exclusion chromatography-multiangle light scattering-refractive index viscometry method. It showed that the EPS was a kind of water-soluble polysaccharide with a moderate molecular weight (34.1 × 104) and a flexible, linear random coil chain structure. The antioxidant activity tests suggested that the EPS has great potential application as a natural antioxidant material in foods and medicines. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,31a39db86881b79c,486c113c0efd1d6a.html