RT Journal Article
ID 486c113c0efd1d6a
A1 Jia, Xuewei
A1 Qu, Lili
A1 Panpan, Ran
A1 Liu, Shuai
A1 Wu, Yan
A1 Xu, Chunping
T1 Characterization and Antioxidant Activity of an Exopolysaccharide Produced by Rigidoporus microporus (Agaricomycetes)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-05-16
VO 20
IS 4
SP 311
OP 320
K1 antioxidant
K1 exopolysaccharide
K1 medicinal mushrooms
K1 optimization
K1 Rigidoporus microporus
AB In this investigation we developed an optimal fermentation medium to produce an exopolysaccharide (EPS) with antioxidant activity from Rigidoporus microporus. Lactose and tryptone were chosen as the optimal carbon and nitrogen sources, respectively, for EPS production, with a 6-day cultivation cycle. After removing proteins through the use of the Sevag method, one EPS fraction was purified from the culture filtrates by gel filtration chromatography on a Sepharose CL-6B column. The preliminary chemical structure of the EPS fraction was determined by Fourier transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance. The results indicated that the main structure of the EPS consisted of β-glucopyranose and mannopyranose. Furthermore, conformational parameters such as molecular weight (Mw), intrinsic viscosity ([η]), mean-square radii of gyration (Rg), and hydrodynamic radius (Rh) of the EPS were characterized using a size exclusion chromatography-multiangle light scattering-refractive index viscometry method. It showed that the EPS was a kind of water-soluble polysaccharide with a moderate molecular weight (34.1 × 104) and a flexible, linear random coil chain structure. The antioxidant activity tests suggested that the EPS has great potential application as a natural antioxidant material in foods and medicines.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,31a39db86881b79c,486c113c0efd1d6a.html