RT Journal Article
ID 4c5e3d861c1bdd9f
A1 Asatiani, Mikheil D.
A1 Wasser, Solomon P.
A1 Nevo, Eviatar D.
A1 Ruimi, Nili
A1 Mahajna, Jamal A.
A1 Reznick, Abraham
T1 The Shaggy Inc Cap Medicinal Mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) Substances Interfere with H2O2 Induction of the NF-κB Pathway through Inhibition of IκBα Phosphorylation in MCF7 Breast Cancer Cells
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2011
FD 2011-03-22
VO 13
IS 1
SP 19
OP 25
K1 medicinal mushrooms
K1 Coprinus comatus substances
K1 IΚBα phosphorylation
K1 NF-ΚB activation pathway
K1 IKK complex
AB Breast cancer is the most commonly diagnosed cancer among women. Currently, there is no effective therapy for malignant estrogen-independent breast cancer. In our study, we used hydrogen peroxide, a well-known strong oxidative reagent capable of activating the nuclear factor kappa B (NF-κB) transcription factor. The IC50 value of the culinary-medicinal Shaggy Inc Cap mushroom Coprinus comatus culture liquid crude extract on MCF7 cell viability was found to be as low as 76 μg/mL, and the IC50 value of C. comatus ethyl acetate extract was only 32 μg/ mL. Our results also showed that both extracts significantly affected IκBα phosphorylation in a dose-dependent manner. The effect of ethyl acetate extract was comparable to the effect of curcumin, a known NF-κB pathway inhibitor, and seemed to be the most active inhibitor of H2O2-dependent IκBα phosphorylation. In addition, the data obtained showed that only ethyl acetate extract inhibited the activity of IKK complex, at close to 90% as compared to the control of the untreated sample. These results suggest that C. comatus contains potent compounds capable of inhibiting NF-κB function and also possibly acts as an antitumor agent.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,77c52f4810e4b47b,4c5e3d861c1bdd9f.html