RT Journal Article ID 58c6dcac49f2fc14 A1 Yao, Chun A1 Dai, Yi-chen A1 Chen, Zhang-xing A1 Xie, Jun-pei A1 Zeng, Wei A1 Lin, Yuan-yuan A1 Tan, Qiu-hui T1 Knockdown of ECHS1 Protein Expression Inhibits Hepatocellular Carcinoma Cell Proliferation via Suppression of Akt Activity JF Critical Reviews™ in Eukaryotic Gene Expression JO CRE YR 2013 FD 2013-07-10 VO 23 IS 3 SP 275 OP 282 K1 ECHS1 K1 HepG2 cells K1 PI3K/Akt AB Overexpression of ECHS1 occurs in different cancers, including hepatocellular carcinoma (HCC). ECHS1 is also reported to have an oncogenic activity in various human cancers. This study investigated the effect of ECHS1 knockdown on the regulation of HCC growth. ECHS1 shRNA suppressed the expression of ECHS1 protein in HepG2 cells compared to the negative control vector-transfected HCC cells. ECHS1 knockdown also reduced HCC cell viability and enhanced cisplatin-induced apoptosis in HCC cells. Akt activation and the expression of various cell cycle-related genes were inhibited following ECHS1 knockdown. ECHS1 shRNA suppressed hepatocellular carcinoma growth in tumor xenograft mice. These data demonstrate that ECHS1 may play a role in HCC progression, suggesting that inhibition of ECHS1 expression using ECHS1 shRNA should be further evaluated as a novel target for the control of HCC. PB Begell House LK https://www.dl.begellhouse.com/journals/6dbf508d3b17c437,3164bc9c570aa2af,58c6dcac49f2fc14.html