RT Journal Article ID 5988a93154e863c9 A1 Bharati, Sanjay A1 Rishi, Parveen A1 Koul, Ashwani T1 Fourier Transform Infrared Spectroscopic Studies on Modulation of N-Nitrosodiethylamine-Induced Hepatocarcinogenesis by Azadirachta indica JF Journal of Environmental Pathology, Toxicology and Oncology JO JEP(T) YR 2015 FD 2015-06-23 VO 34 IS 3 SP 227 OP 236 K1 chemoprevention K1 carcinogen metabolism K1 hepatic tumors K1 hepatocellular carcinoma AB Fourier transform infrared spectroscopy was employed in the present study to obtain information about the molecular composition of hepatic tumor versus hepatic tissue. A hepatic cancer model was developed by administering N-nitrosodiethylamine (NDEA) to male Balb/c mice. The results revealed that NDEA-induced hepatic cancer tumor tissue had altered molecular composition compared with normal liver tissue. Compared with the normal tissue, the saturation level of membrane phospholipids was observed to be decreased in tumors along with an abnormal distribution of protein secondary structures. A significant decrease in glycogen and a significant increase in total nucleic acid content were also observed in tumor cells. The administration of aqueous Azadirachta indica leaf extract (AAILE) prior to NDEA treatment resulted in the normalization of saturation levels in phospholipids and total nucleic acid content and in the distribution of protein secondary structures in tumors. A significant increase in the amount of stored glycogen was observed in AAILE cotreated tumors compared with NDEA-induced tumors, which might indicate that AAILE cotreatment impeded the ability of tumor cells to consume glucose at a faster rate. The normalization of molecular composition upon AAILE cotreatment in hepatic tumors might indicate that AAILE hampered the process of evolution of tumors, which could be responsible for its observed chemopreventive action. PB Begell House LK https://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,5e769fe6726f83d4,5988a93154e863c9.html