RT Journal Article
ID 61f0f56f26707937
A1 Liaw, Chih-Chuang
A1 Wu, Shu-Jing
A1 Chen, Ching-Fu
A1 Lai, Ming-Nan
A1 Ng, Lean-Teik
T1 Anti-Inflammatory Activity and Bioactive Constituents of Cultivated Fruiting Bodies of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2017
FD 2017-11-29
VO 19
IS 10
SP 915
OP 924
K1 anti-inflammatory
K1 bioactive compounds
K1 medicinal mushroom
K1 Xylaria nigripes
AB Xylaria nigripes, also known as Wu Ling Shen, is popular for treating insomnia and trauma in traditional Chinese medicine. This study aimed to examine the anti-inflammatory activity and bioactive constituents of cultivated X. nigripes fruiting bodies in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Results showed that among the different extracts, the hexane fraction exhibited the best protection against cell toxicity induced by 1 μg/mL LPS and the strongest inhibitory effect on nitric oxide (NO) production. This fraction led to the isolation of 2 bioactive compounds (namely, XN-CP1 and XN-CP2), which were confirmed to be ergostarien-3β-ol and ergosterol peroxide, respectively. Although both XN-CP1 and XN-CP2 showed good inhibitory effects on NO, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and prostaglandin E2 production in LPS-stimulated macrophages, XN-CP2 was shown to have a stronger anti-inflammatory activity; this was further supported by its strong suppressive effects on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression and nuclear factor (NF)-κB activation. These results conclude that ergosterol peroxide (XN-CP2) could be the main bioactive compound contributing to the potent anti-inflammatory activity of X. nigripes, and its mechanism of action is mediated through inhibition of iNOS and COX-2 expression via the NF-κB signaling pathway.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,305c54e9703680c8,61f0f56f26707937.html