RT Journal Article ID 69a1adc30c7d75f1 A1 Sovrani, Vanessa A1 da Rosa, Juliana A1 Drewinski, Mariana de Paula A1 Colodi, Franciely Grose A1 Tominaga, Tânia Toyomi A1 Santa, Herta Stutz Dalla A1 Rebeca, Rosilene T1 In Vitro and In Vivo Antitumoral Activity of Exobiopolymers from the Royal Sun Culinary-Medicinal Mushroom Agaricus brasiliensis (Agaricomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2017 FD 2017-11-01 VO 19 IS 9 SP 767 OP 775 K1 Agaricus brasiliensis K1 exobiopolymers K1 exopolysaccharide K1 HeLa K1 HEp-2 K1 medicinal mushrooms K1 Walker 256 tumor AB We studied antitumor activity in vitro and in vivo and partial chemical characterization of exobiopolymers (EBPs) of Agaricus brasiliensis produced by submerged cultivation. The EBPs comprised 61.2% total sugar and 22.3% total protein, and the chromatographic profile mainly identified mannose (65.5%), galactose (17.5%), and glucose (13.1%). In vitro analysis revealed a dose with antitumor effectiveness, time-dependent treatment, and 46% inhibition of solid Walker 256 tumors in rats. Metabolic changes were significant in the spleens of the animals; this is possibly related to higher immune activity triggered by the presence of EBPs in the treated group. The increase of nitric oxide production by peritoneal macrophages from animals may be related to the tumor reduction observed, because nitric oxide acts as an important effector pathway in the defense of the organism. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,53402a884df3ae15,69a1adc30c7d75f1.html