%0 Journal Article %A Klimaszewska, Marzenna %A Górska, Sandra %A Dawidowski, Maciej %A Podsadni, Piotr %A Szczepańska, Agnieszka %A Orzechowska, Emilia %A Kurpios-Piec, Dagmara %A Grosicka-Maciag, Emilia %A Rahden-Staroń, Iwonna %A Turło, Jadwiga %D 2017 %I Begell House %K chemoprevention, Lentinus edodes, medicinal mushrooms, Se-methyl-seleno-L-cysteine, selenium, selenium-containing polysaccharides %N 8 %P 709-716 %R 10.1615/IntJMedMushrooms.2017021250 %T Selective Cytotoxic Activity of Se-Methyl-Seleno-L-Cysteine– and Se-Polysaccharide–Containing Extracts from Shiitake Medicinal Mushroom, Lentinus edodes (Agaricomycetes) %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,32c8651274405055,6dc7ca1b3fba21c0.html %V 19 %X Numerous formulations derived from the shiitake medicinal mushroom, Lentinus edodes, demonstrate anticancer activities. We hypothesized that isolates from selenium (Se)-enriched mycelia of L. edodes would possess stronger cancer-preventive properties than current preparations. The aim of this study was to investigate whether the presence of Se-methyl-seleno-L-cysteine in mycelial extracts of L. edodes affects their cytotoxic activity (makes them stronger) or whether they are as effective as Se-containing polysaccharides. Extracts were prepared from Se-containing mycelia under various conditions and assayed for cytotoxic activity in cancer (PC3 and HeLa) and normal (HMEC-1) cell lines. The chemical composition of the extracts was examined; specifically, the amounts of potentially cytotoxic Se compounds (methylselenocysteine, selenomethionine, and Se-containing polysaccharides) were measured. The relationship between extract composition and biological activity was characterized. Mycelial cultures were cultivated in a 10-L bioreactor in medium enriched with sodium selenite. Mycelial extracts were prepared either at 100°C or at 4°C in acidic solution. Total Se content was determined using the atomic absorption spectrometry method, and methylselenocysteine and selenomethionine contents were measured using reverse-phase high-performance liquid chromatography. Protein, carbohydrate, and polyphenolic contents were determined with spectrophotometric methods, and Se-containing polysaccharides were measured with the use of precipitation. Anticancer activity of mycelial extracts was examined using the MTT cell viability assay. Extracts containing Se-methyl-seleno-L-cysteine or Se-polysaccharides prepared at 4°C and 100°C, respectively, display moderate, time-dependent, specific cytotoxic activity in HeLa and PC3 cell lines. The effect in HeLa cells is more pronounced in the extract prepared at 4°C than at 100°C. The effect is almost equal for the PC3 cell line. However, both extracts have no effect or only slightly stimulate normal (HMEC-1) cell viability. The selective cytotoxic activity of L. edodes extracts in cancer (PC3 and HeLa) cells is due to the presence of both Se-methyl-seleno-L-cysteine and selenated polysaccharides, perhaps in combination with other active ingredients. %8 2017-10-16