RT Journal Article ID 70a0e3bc21241e53 A1 Panasiuk, Olga S. A1 Shysh, Angela M. A1 Dosenko, Viktor E. A1 Moibenko, Oleksiy O. T1 Omega-3 Polyunsaturated Fatty Acids Normalize the Functions of Mitochondria, Pro- and Antioxidant Enzymes of, and Cytochrome P450 2E1 Expression after Isoproterenol-Induced Myocardial Injury JF International Journal of Physiology and Pathophysiology JO IJPP YR 2017 FD 2017-08-24 VO 8 IS 2 SP 131 OP 139 K1 mitochondria K1 subsarcolemmal fraction K1 interfibrillar fraction K1 isoproterenol K1 w-3 polyunsaturated fatty acid K1 malondialdehyde K1 superoxide dismutase K1 catalase K1 cytochrome P450 2E1 AB We studied the effect of dietary ω-3 polyunsaturated fatty acids (ω-3 PUFA) on the subsarcolemmal and interfibrillar mitochondrial fractions of rat myocardium, changes in expression of cytochrome P450 (CYP2E1), and the activity of pro-antioxidant enzymes after isoproterenol-induced myocardial injury. It has been found that ω-3 PUFA (Epadol 0.1 ml/100 g for 4 weeks) significantly reduces the swelling of the subsarcolemmal and interfibrillar mitochondrial fractions by 65.52% and 54.84%, respectively, indicating a decrease in damage to the mitochondrial function during isoproterenol-induced injury (two daily subcutaneous injections of isoproterenol at the dose of 60 mg/kg). In case of isoproterenol-induced myocardial injury, the use of ω-3 PUFAs prevents a decrease in the activity of antioxidant enzymes, namely catalase and superoxide dismutase (2.65 and 7.1 times, respectively). We have revealed that the development of oxidative stress after isoproterenol-induced myocardial injury can be triggered by a significant increase in the expression of cytochrome P450 2E1 (73.3%), and applying of ω-3 PUFAs prevents such changes. PB Begell House LK https://www.dl.begellhouse.com/journals/6ec4ba27650016b1,4a1e5fe11470826b,70a0e3bc21241e53.html