RT Journal Article ID 7b52359d34386032 A1 Chen, Jian-Zhi A1 Lo, Hui-Chen A1 Lin, Fang-Yi A1 Chang, Shih-Liang A1 Hsieh, Chang-Wei A1 Liang, Zeng-Chin A1 Ho, Wai-Jane A1 Hsu, Tai-Hao T1 Effects of Medium Components and Culture Conditions on Mycelial Biomass and the Production of Bioactive Ingredients in Submerged Culture of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus JF International Journal of Medicinal Mushrooms JO IJM YR 2014 FD 2014-09-12 VO 16 IS 5 SP 431 OP 447 K1 medicinal mushrooms and fungi K1 Xylaria nigripes K1 submerged cultivation K1 mycelial biomass K1 bioactive ingredients AB The optimal culture conditions were investigated to maximize the production of mycelial biomass and bioactive ingredients in submerged cultivation of Xylaria nigripes, a Chinese medicinal fungus. The one-factor-at-a-time method was used to explore the effects of medium components, including carbon, nitrogen, mineral sources, and initial pH of the medium and environmental factors, such as culture temperature and rotation speed, on mycelial growth and production of bioactive ingredients. The results indicated that the optimal culture temperature and rotation speed were 25°C and 100 rpm in a medium with 20 g fructose, 6 g yeast extract, and 2 g magnesiun sulfate heptahydrate as carbon, nitrogen, and mineral sources, respectively, in 1 L distilled water with an initial medium pH of 5.5. With optimal medium components and conditions of cultivation, the maximal production of mycelial biomass was 6.64 ± 0.88 g/L, with maximal production of bioactive ingredients such as extracellular polysaccharides (2.36 ± 0.18 mg/mL), intracellular polysaccharides (2.38 ± 0.07 mg/g), adenosine (43.27 ± 2.37 mg/g), total polyphenols (36.57 ± 1.36 mg/g), and triterpenoids (31.29 ± 1.17 mg/g) in a shake flask culture. These results suggest that different bioactive ingredients including intracellular polysaccharides, adenosine, total polyphenols and triterpenoids in mycelia and extracellular polysaccharides in broth can be obtained from one simple medium for submerged cultivation of X. nigripes. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,266d4152107fca7a,7b52359d34386032.html