Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
Journal of Environmental Pathology, Toxicology and Oncology
Impact-faktor: 1.241 5-jähriger Impact-Faktor: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Druckformat: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v20.i1.110
11 pages

Low Doses of Heavy Metals Disrupt Normal Structure and Function of Rat Platelets

Siragam Vinaya Kumar
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India
Rakhi Bose
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India
Shelley Bhattacharya
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India

ABSTRAKT

Aggregation, an important property of platelets, plays a key role in the coagulation of blood and is potentiated by ADP and adrenaline, whereas cAMP acts as a strong inhibitor. Because of the rise in the heavy metal load in the environment, more studies are necessary to look at its subtle effects in the animal system, wherein platelet structure and function can be targeted. We carried out structural analyses under phase contrast and scanning electronmicroscopy of the platelets prepared from 1/10 LD50 metal-treated rats. After mercury (Hg) and arsenic (As) treatment, the cell margins appeared irregular and wavy, with small pseudopodia-like protrusions from the surface. Cadmium (Cd) treatment caused loss of the general spindle shape, and the platelets assumed a round spongy appearance. All metal treatments effected enhanced collagen-induced aggregation and inhibited ADP and arachidonic acid-induced aggregation, whereas epinephrine accelerated aggregation in Hg and Cd treatment but inhibited aggregation in As treatment. Cd proved a potent inhibitor of phosphodiesterase, increasing the cAMP level in in vitro treatments at equimolar (5, 10, and 20 pmoles) concentrations. The rate of aggregation was enhanced with all the agonists used in in vitro Hg and As treatments, with concomitant reduction in cAMP, while Cd inhibited platelet aggregation. Thus, we can conclude that cytosolic cAMP, which is decreased by the metal-induced inhibition of phosphodiesterase, is a regulator molecule in platelet aggregation. Furthermore, it is also abundantly clear that equimolar doses of metals are not always equitoxic. Therefore, the action of each xenobiotic is not only due to the concentration used but also to the type of xenobiotic depending on its unique mechanism of action.


Articles with similar content:

Inhibition of Epithelial-to-Mesenchymal Transition (EMT) in Cancer by Nitric Oxide: Pivotal Roles of Nitrosylation of NF-κB, YY1 and Snail
Forum on Immunopathological Diseases and Therapeutics, Vol.3, 2012, issue 2
Stavroula Baritaki, Benjamin Bonavida
Effect of Amentoflavone, a Phenolic Component From Biophytum sensitivum, on Cell Cycling and Apoptosis of B16F-10 Melanoma Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 4
K.S. Siveen , Girija Kuttan
Hot Water Extracts of the Royal Sun Mushroom, Agaricus brasiliensis (Higher Basidiomycetes), Inhibit Platelet Activation via the P2Y1 Receptor
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 8
Kaneo Satoh, Katsuyuki Akahane, Yukio Ozaki, Masato Ohta
Effects of Cytokine-Like Polypeptide EMAP II and Flutamide on Testosterone-Stimulated Prostate of Castrated Rats
International Journal of Physiology and Pathophysiology, Vol.3, 2012, issue 2
Olga V. Sachynska, L. V. Chaikovskaya, L. I. Polyakova, Alexander G. Reznikov
Epithelial-Mesenchymal Transition: A Special Focus on Phthalates and Bisphenol A
Journal of Environmental Pathology, Toxicology and Oncology, Vol.35, 2016, issue 1
Belmar Kocer-Gumusel, Ming-Wei Chao, Didem Oral, Pinar Erkekoglu