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ISSN Druckformat: 0731-8898
ISSN Online: 2162-6537
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A Clinical Investigation to Determine the Effect of Pressure Injection on the Penetration of Topical Methyl Aminolevulinate into Nodular Basal Cell Carcinoma of the Skin
ABSTRAKT
This investigation considered a novel method of enhancing penetration of the topical photosensitizing agent methyl aminolevulinate (MAL) into nodular basal cell carcinomas (BCCs) using an oxygen pressure injection device. Oxygen pressure injection (OPI) is a method to drive compounds into skin using pressured oxygen. The study was an observer-blinded pilot of a single application of MAL to nBCCs, with or without the use of OPI. The BCCs were then excised at different time intervals (0−180 min) and the depth of penetration of the MAL examined using microscopic fluorescence photometry to detect the production of the naturally fluorescent active photosensitiser protoporphyrin IX (PpIX). A highly selective and homogeneous distribution of MAL-induced porphyrin fluorescence was seen in all nBCC tumors studied, and showed a high lesion-to-normal-tissue ratio with very little fluorescence in the surrounding normal tissue. Although it was difficult to compare quantitatively, as individual tumors in each of the different study groups varied, a definite trend of increase in relative tumor concentration of MAL-induced PpIX was observed over time, and this was enhanced when OPI was employed.
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Blake E., Campbell S., Allen J., Mathew J., Helliwell P., Curnow A., The time-dependent accumulation of protoporphyrin IX fluorescence in nodular basal cell carcinoma following application of methyl aminolevulinate with an oxygen pressure injection device, Journal of Photochemistry and Photobiology B: Biology, 117, 2012. Crossref
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Blake E., Allen J., Thorn C., Shore A., Curnow A., Effect of an oxygen pressure injection (OPI) device on the oxygen saturation of patients during dermatological methyl aminolevulinate photodynamic therapy, Lasers in Medical Science, 28, 3, 2013. Crossref
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Campbell S.M., Morton C.A., Alyahya R., Horton S., Pye A., Curnow A., Clinical investigation of the novel iron-chelating agent, CP94, to enhance topical photodynamic therapy of nodular basal cell carcinoma, British Journal of Dermatology, 159, 2, 2008. Crossref
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Garg Tarun, Rath Goutam, Goyal Amit K., Comprehensive review on additives of topical dosage forms for drug delivery, Drug Delivery, 22, 8, 2015. Crossref
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Tyrrell Jessica, Campbell Sandra M., Curnow Alison, Monitoring the accumulation and dissipation of the photosensitizer protoporphyrin IX during standard dermatological methyl-aminolevulinate photodynamic therapy utilizing non-invasive fluorescence imaging and quantification, Photodiagnosis and Photodynamic Therapy, 8, 1, 2011. Crossref
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Schmitz Lutz, Novak Ben, Hoeh Ann-Kathrin, Luebbert Herman, Dirschka Thomas, Epidermal penetration and protoporphyrin IX formation of two different 5-aminolevulinic acid formulations in ex vivo human skin, Photodiagnosis and Photodynamic Therapy, 14, 2016. Crossref
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Heuck Gesine, Lange Norbert, Exogenously Induced Endogenous Photosensitizers, in Photosensitizers in Medicine, Environment, and Security, 2011. Crossref