Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
Journal of Environmental Pathology, Toxicology and Oncology
Impact-faktor: 1.625 5-jähriger Impact-Faktor: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN Druckformat: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v28.i4.90
pages 351-359

Role of Zinc in Modulating Histoarchitectural and Biochemical Alterations During Dimethylhydrazine (DMH)-Induced Rat Colon Carcinogenesis

Anshoo Malhotra
Post Graduate Institute of Medial Education and Research, India
Vijayta Dani Chadha
Centre for Nuclear Medicine, Institute for Emerging Ares in Science and Technology, and Department of Biophysics, Panjab University, Chandigarh, India
Praveen Nair
Department of Biophysics, Panjab University, Chandigarh, India
Devinder K. Dhawan
Department of Biophysics and Centre of Nuclear Medicine, Institute for Emerging Areas in Science and Technology, Panjab University, Chandigarh, India

ABSTRAKT

The aim of the present work was to gain insight into the putative anticancer effect of dietary zinc during 1,2 dimethylhydrazine (DMH)-induced colon carcinogenesis. The rats were segregated into four groups, namely, normal control, DMH-treated, zinc-treated, and (DMH + zinc)-treated. Colon carcinogenesis was induced through weekly subcutaneous injections of DMH (30 mg/kg body weight) for 12 weeks. Zinc in the form of zinc sulfate was supplemented to rats at a dose level of 227 mg/L in drinking water, ad libitum for the entire duration of the study. The effects of different treatments were studied on lipid peroxidation (LPO), reduced glutathione (GSH), and anitioxidative enzymes, which included superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione reductase (GR), as well as on the histoarchitecture of the colon. A total of 12 weeks of DMH treatment resulted in a significant increase in LPO. GSH levels and the activities of SOD, CAT, and GST were found to be significantly decreased following DMH treatment. A significant elevation in the activity of GR was observed following 12 weeks of DMH treatment. Histopathological studies showed well-differentiated signs of dysplasia, which included nuclei enlargement, epithelial thickening, and nuclear pleomorphism indicative of promotional phase of colon carcinogenesis in DMH-administered rats. Administration of zinc to DMH-treated rats decreased the levels of LPO and GSH significantly, but the activities of SOD and CAT were found to be significantly increased following zinc treatment. Zinc supplementation along with DMH treatment did not reveal any significant change in the activity of GR but significantly improved the activity of GST, which was depressed following DMH treatment. Also, zinc treatment in DMH-treated rats showed signs of great improvement, but structureless masses of the cells and hyperchromic nuclei were still visible occasionally. In conclusion, the results of this study suggest that zinc has a positive beneficial effect against chemically DMH-induced colonic preneoplastic progression in rats.


Articles with similar content:

Protective Role of Zinc in Ameliorating Arsenic-Induced Oxidative Stress and Histological Changes in Rat Liver
Journal of Environmental Pathology, Toxicology and Oncology, Vol.29, 2010, issue 2
Devinder K. Dhawan, Anshoo Malhotra, Ashok Kumar, Praveen Nair, M. L. Garg
Efficacy of Zinc as a Nutritional Supplement in Ameliorating Chlorpyrifos-Induced Neurotoxicity in Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 3
Devinder K. Dhawan, Anshoo Malhotra, Praveen Nair
In Vitro 14C-Labeled Amino Acid Uptake Changes and Surface Abnormalities in the Colon after 1,2-Dimethylhydrazine—Induced Experimental Carcinogenesis: Protection by Zinc
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 2
Devinder K. Dhawan, Vijayta Dani Chadha
Efficacy of Curcumin in Ameliorating Aluminum- Induced Neurotoxicity
Journal of Environmental Pathology, Toxicology and Oncology, Vol.37, 2018, issue 2
Hongyuan Zhang, Wei Zhao, Anshoo Malhotra
Naringenin Ameliorates Doxorubicin Toxicity and Hypoxic Condition in Dalton's Lymphoma Ascites Tumor Mouse Model: Evidence from Electron Paramagnetic Resonance Imaging
Journal of Environmental Pathology, Toxicology and Oncology, Vol.35, 2016, issue 3
Kumaresan Ganesan, Venkatesan Kathiresan, Murugesan Ramachandran, Swathika Subburaman, Mathivanan Natarajan, Arun Venkatesh Krishna, Gandhidasan Rathinasamy