Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
Journal of Environmental Pathology, Toxicology and Oncology
Impact-faktor: 1.241 5-jähriger Impact-Faktor: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Druckformat: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvPathToxOncol.v23.i3.50
12 pages

Prevention and Repair of DNA Damage by Selected Phytochemicals as Measured by Single Cell Gel Electrophoresis

Sutapa Chakraborty
Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, Kolkata, India
Madhumita Roy
Department of Environmental Carcinogenesis & Toxicology, Chittaranjan National Cancer Institute, Calcutta, India
Rathindra Kumar Bhattacharya
Department of Environmental Carcinogenesis & Toxicology, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700026

ABSTRAKT

We assessed the ability of some natural products−namely, curcumin, resveratrol, indole-3-carbinol, and ellagic acid−to modify the DNA damaging ability of the alkylating carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in cultured Chinese hamster lung fibroblast cells (CH V-79). MNNG produced DNA single strand breaks in a dose- and time-dependent manner, as observed by increase in the tail moments of the comet, when the cells were subjected to alkaline single cell gel electrophoresis. When the cells were treated in the presence of each of the natural compounds, the DNA damage caused by MNNG was considerably reduced. This effect was found to be dose related. Preincubation of cells with each of these compounds individually afforded significant protection to DNA against damage caused by subsequent treatment with MNNG, indicating a true chemopreventive role of these substances. The most remarkable aspect of the present study was that all four compounds helped in the recovery of DNA damage by accelerating DNA repair efficiency in the damaged cells. This was further substantiated by the observation on unscheduled DNA synthesis. Our results suggest that these agents are chemopreventive by virtue of their ability to protect DNA as well as to induce DNA repair.


Articles with similar content:

Biochanin A Enhances the Radiotoxicity in Colon Tumor Cells In Vitro
Journal of Environmental Pathology, Toxicology and Oncology, Vol.32, 2013, issue 3
Reeta Tiwari, Abhay Puthli, Kaushala Prasad Mishra
Evaluation of Radioprotective Action of Compounds Using Saccharomyces cerevisiae
Journal of Environmental Pathology, Toxicology and Oncology, Vol.23, 2004, issue 2
Bishnavath K. Chourasia, Purva Nemavarkar, Karpagam Pasupathy
Curative Effect of Amorphophallus campanulatus (Roxb.) Blume. Tuber On N-Nitrosodiethylamine- Induced Hepatocellular Carcinoma in Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.33, 2014, issue 3
Santhibhavan Prabhakaran Prabha, Anand Nitha, Puthuparampil Nazarudeen Ansil, Mukalel Sankunni Latha
The Cyclooxygenase-2 Inhibitor Etoricoxib Is a Potent Chemopreventive Agent of Colon Carcinogenesis in the Rat Model
Journal of Environmental Pathology, Toxicology and Oncology, Vol.28, 2009, issue 1
Sankar Nath Sanyal, Manpreet Kaur Saini, Pinky Sharma, Jasmeet Kaur
Abrogation of Thioacetamide-Induced Biochemical Events of Hepatic Tumor Promotion Stage by Tannic Acid in Wistar Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.26, 2007, issue 1
Sarwat Sultana, Anuradha Sehrawat