Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
Journal of Environmental Pathology, Toxicology and Oncology
Impact-faktor: 1.241 5-jähriger Impact-Faktor: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Druckformat: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvPathToxOncol.v24.i1.40
pages 33-44

Cell Cycle Regulators Modulating Con A Mitogenesis and Apoptosis in Low-Dose Radiation-Exposed Mice

Bhavani Shankar
Immunology Section, Radiation Biology Health Sciences Division, Bhabha Atomic Research Centre, Modular Laboratories, Trombay, Mumbai, India
Krishna B. Sainis
Immunology Section, Radiation Biology Health Sciences Division, Bhabha Atomic Research Centre, Modular Laboratories, Trombay, Mumbai, India

ABSTRAKT

Low doses of ionizing radiation (LDR) are reported to induce transient changes in mouse lymphocytes, such as enhanced response to polyclonal T cell mitogens, increased expression of heat shock proteins, and p53. We evaluated the role of cell cycle proteins and apoptosis in lymphocytes of C57BL/6 mice exposed to 20 cGy fractionated LDR. We found an enhanced cell proliferation in response to the mitogen concanavalin A (con A). The expression of several cell cycle and apoptosis-related intracellular and extracellular proteins was analyzed by flowcytometry following labeling with specific antibodies. An increased response to con A was accompanied by an increase in the expression of proliferating cell nuclear antigen (PCNA) and cyclins D and A. The expression of cyclin B did not change significantly. In 20 cGy-exposed C57BL/6 mice, the caspase activity and apoptosis were reduced in con A-stimulated spleen cells as compared to sham controls. The expression of Fas and Fas ligand was analyzed by labeling with specific antibodies followed by flowcytometry. There was no change in the expression of Fas and Fas ligand. The change in the mitochondrial transmembrane potential was followed by labeling the cells with the dye 5,5',6,6',-tetrachloro-1,1,3,3-tetraethylbenzimidazolcarbocyanine iodide (JC-I) and analyzing by flowcytometry. Mitochondrial stability was increased in spleen cells of LDR-treated mice. These data suggest that LDR induces augmentation of mitogenic response by modulation of expression of cyclins and the mitochondrial membrane potential leading to reduced apoptosis.


Articles with similar content:

Vernolide-A Inhibits Radiation-Induced Hypoxia-Mediated Tumor Angiogenesis by Regulating HIF-1α, MMP-2, MMP-9, and VEGF
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 2
P. Pratheeshkumar, Girija Kuttan
Mechanisms Mediating Oxalate-Induced Alterations in Renal Cell Functions
Critical Reviews™ in Eukaryotic Gene Expression, Vol.13, 2003, issue 1
Thomas Honeyman, Julie A. Jonassen, Cheryl R. Scheid, Lu-Cheng Cao
Chemoprevention of Colon Cancer through Inhibition of Angiogenesis and Induction of Apoptosis by Nonsteroidal Anti-Inflammatory Drugs
Journal of Environmental Pathology, Toxicology and Oncology, Vol.35, 2016, issue 3
Sankar Nath Sanyal, Preety Ghanghas, Chandan Rana, Shelly Jain
Role of Cannabinoid and Vanilloid Receptors in Invasion of Human Breast Carcinoma Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.31, 2012, issue 4
N. Farsandaj, Seyed Nasser Ostad, Mohammad Hossein Ghahremani
Oleanolic acid induces Apoptosis by modulating p53, Bax, Bcl-2 and caspase-3 gene expression and regulates the activation of transcription factors and cytokine profile in B16F
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 1
P. Pratheeshkumar, Girija Kuttan