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Forum on Immunopathological Diseases and Therapeutics
SJR: 0.164 SNIP: 0.041 CiteScore™: 0.18

ISSN Druckformat: 2151-8017
ISSN Online: 2151-8025

Archives: Volume 1, 2010 to Volume 7, 2016

Forum on Immunopathological Diseases and Therapeutics

DOI: 10.1615/ForumImmunDisTher.2011004235
pages 237-246

Photodynamic Activity of Nanogold-Doped Fotolon: Free Radicals Versus Singlet Oxygen

Nikolai Gamaleia
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine
Genadiy Dolinsky
R.E. Kavetsky Institute for Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Ukraine
Eugenia Shishko
R.E. Kavetsky Institute for Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Ukraine
Alexander Shcherbakov
Research Institute for Nanotechnological Industry, Ukraine
Alexander Usatenko
Research Institute for Nanotechnological Industry, Ukraine
Irina Shton
R.E. Kavetsky Institute for Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Ukraine
Petro Yermak
R.E. Kavetsky Institute for Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Ukraine

ABSTRAKT

Photodynamic therapy (PDT) is a modern, selective, and noninvasive method of treating tumors, but its practical application is limited by poor penetration of light into biological tissues. Photosensitizers, combined with nanoparticles serving as a transportation vector, accumulate better in tumor tissues and make them more light responsive, even to scarce light penetration. We prepared a combined photosensitizer from Fotolon (chlorin e6) doped with gold nanoparticles (GNP). The GNP-doped Fotolon showed higher photodynamic activity for MT-4 cell line than Fotolon alone. Because singlet oxygen (1O2) and free radicals are considered to be the main effectors of PDT, we attempted to evaluate a role of these oxidative factors in photodynamic effects of Fotolon in comparison with its GNP-doped analog. 1O2 trapping by sodium azide reduced the increase in cell death, caused by GNP doping. Nevertheless, fluorescence assay with an 1O2 -specific sensor revealed a decrease in 1O2 production by GNP-doped Fotolon. To resolve the contradiction, further investigation of intrinsic Fotolon fluorescence and free radical oxidation-derived chemiluminescence in phospholipid model system were performed. Our results support the primacy of the PDT type II mechanism with a subsequent increase in secondary free-radical generation through GNP catalytic activity.


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