Erscheint 6 Ausgaben pro Jahr
ISSN Druckformat: 0743-4863
ISSN Online: 2162-660X
Indexed in
Spray-Dried Powders for Pulmonary Drug Delivery
ABSTRAKT
Powders for inhalation are traditionally prepared using a destructive micronization process such as jet milling to reduce the particle size of the drug to 2−5 μm. The resultant particles are typically highly cohesive and display poor aerosolization properties, necessitating the addition of a coarse carrier particle to the micronized drug to improve powder flowability. Spray-drying technology offers an alternative, constructive particle production technique to the traditional destructive approach, which may be particularly useful when processing biotechnology products that could be adversely affected by high-energy micronization processes. Advantages of spray drying include the ability to incorporate a wide range of excipients into the spray-drying feedstock, which could modify the aerosolization and stability characterizations of the resultant powders, as well as modify the drug release and absorption profiles following inhalation. This review discusses some of the reasons why pulmonary drug delivery is becoming an increasingly popular route of administration and describes the various investigations that have been undertaken in the preparation of spray-dried powders for pulmonary drug delivery.
-
Yang Jing-Jing, Liu Chun-Yu, Quan Li-Hui, Liao Yong-Hong, Preparation and In Vitro Aerosol Performance of Spray-Dried Shuang-Huang-Lian Corrugated Particles in Carrier-Based Dry Powder Inhalers, AAPS PharmSciTech, 13, 3, 2012. Crossref
-
Xu Zhen, Mansour Heidi M., Hickey Anthony J., Particle Interactions in Dry Powder Inhaler Unit Processes: A Review, Journal of Adhesion Science and Technology, 25, 4-5, 2011. Crossref
-
Ingvarsson Pall Thor, Yang Mingshi, Mulvad Helle, Nielsen Hanne Mørck, Rantanen Jukka, Foged Camilla, Engineering of an Inhalable DDA/TDB Liposomal Adjuvant: A Quality-by-Design Approach Towards Optimization of the Spray Drying Process, Pharmaceutical Research, 30, 11, 2013. Crossref
-
Colombo Gaia, Parlati Chiara, Russo Paola, Pharmaceutical development studies for inhalation products, in Inhalation Drug Delivery, 2013. Crossref
-
Miladi K., Sfar S., Fessi H., Elaissari A., Drug carriers in osteoporosis: Preparation, drug encapsulation and applications, International Journal of Pharmaceutics, 445, 1-2, 2013. Crossref
-
Cruz Letícia, Fattal Elias, Tasso Leandro, Freitas Gabrielle C., Carregaro Adriano B., Guterres Sílvia S., Pohlmann Adriana R., Tsapis Nicolas, Formulation and in vivo evaluation of sodium alendronate spray-dried microparticles intended for lung delivery, Journal of Controlled Release, 152, 3, 2011. Crossref
-
Cipolla David, Shekunov Boris, Blanchard Jim, Hickey Anthony, Lipid-based carriers for pulmonary products: Preclinical development and case studies in humans, Advanced Drug Delivery Reviews, 75, 2014. Crossref
-
Beck-Broichsitter Moritz, Schweiger Christoph, Schmehl Thomas, Gessler Tobias, Seeger Werner, Kissel Thomas, Characterization of novel spray-dried polymeric particles for controlled pulmonary drug delivery, Journal of Controlled Release, 158, 2, 2012. Crossref
-
Parlati Chiara, Colombo Paolo, Buttini Francesca, Young Paul M., Adi Handoko, Ammit Alaina J., Traini Daniela, Pulmonary Spray Dried Powders of Tobramycin Containing Sodium Stearate to Improve Aerosolization Efficiency, Pharmaceutical Research, 26, 5, 2009. Crossref
-
Weers Jeffry G., Bell John, Chan Hak-Kim, Cipolla David, Dunbar Craig, Hickey Anthony J., Smith Ian J., Pulmonary Formulations: What Remains to be Done?, Journal of Aerosol Medicine and Pulmonary Drug Delivery, 23, S2, 2010. Crossref
-
Salama Rania O., Traini Daniela, Chan Hak-Kim, Sung Adrian, Ammit Alaina J., Young Paul M., Preparation and Evaluation of Controlled Release Microparticles for Respiratory Protein Therapy, Journal of Pharmaceutical Sciences, 98, 8, 2009. Crossref
-
Beck-Broichsitter Moritz, Schmehl Thomas, Seeger Werner, Gessler Tobias, Evaluating the Controlled Release Properties of Inhaled Nanoparticles Using Isolated, Perfused, and Ventilated Lung Models, Journal of Nanomaterials, 2011, 2011. Crossref
-
Zhu Changcheng, Shoji Yoko, McCray Scott, Burke Michael, Hartman Caitlin E., Chichester Jessica A., Breit Jeff, Yusibov Vidadi, Chen Dexiang, Lal Manjari, Stabilization of HAC1 Influenza Vaccine by Spray Drying: Formulation Development and Process Scale-Up, Pharmaceutical Research, 31, 11, 2014. Crossref
-
Merchant Zahra, Taylor Kevin M.G., Stapleton Paul, Razak Sana A., Kunda Nitesh, Alfagih Iman, Sheikh Khalid, Saleem Imran Y., Somavarapu Satyanarayana, Engineering hydrophobically modified chitosan for enhancing the dispersion of respirable microparticles of levofloxacin, European Journal of Pharmaceutics and Biopharmaceutics, 88, 3, 2014. Crossref
-
Cai Cuifang, Wang Lei, Dong Wenna, Tang Xing, Influence of potential inhalation carriers on stability of thymopentin in rat bronchoalveolar lavage fluid, Drug Delivery, 21, 6, 2014. Crossref
-
Aquino R.P., Prota L., Auriemma G., Santoro A., Mencherini T., Colombo G., Russo P., Dry powder inhalers of gentamicin and leucine: formulation parameters, aerosol performance and in vitro toxicity on CuFi1 cells, International Journal of Pharmaceutics, 426, 1-2, 2012. Crossref
-
Yang Yang, Yang Ziwei, Ren Yufeng, Mei Xingguo, Effects of formulation and operating variables on Zanamivir dry powder inhalation characteristics and aerosolization performance, Drug Delivery, 21, 6, 2014. Crossref
-
Razavi Rohani Seyed Salman, Abnous Khalil, Tafaghodi Mohsen, Preparation and characterization of spray-dried powders intended for pulmonary delivery of Insulin with regard to the selection of excipients, International Journal of Pharmaceutics, 465, 1-2, 2014. Crossref
-
Wessman Per, Edwards Katarina, Mahlin Denny, Structural effects caused by spray‐ and freeze‐drying of liposomes and bilayer disks, Journal of Pharmaceutical Sciences, 99, 4, 2010. Crossref
-
Xu En-Yu, Guo Jing, Xu Ying, Li Hao-Ying, Seville Peter C., Influence of excipients on spray-dried powders for inhalation, Powder Technology, 256, 2014. Crossref
-
Beck-Broichsitter Moritz, Merkel Olivia M., Kissel Thomas, Controlled pulmonary drug and gene delivery using polymeric nano-carriers, Journal of Controlled Release, 161, 2, 2012. Crossref
-
Liu Chun, Kong Chao, Wu Guoliang, Zhu Junhao, Javid Babak, Qian Feng, Uniform and amorphous rifampicin microspheres obtained by freezing induced LLPS during lyophilization, International Journal of Pharmaceutics, 495, 1, 2015. Crossref
-
Grenha A., Al-Qadi S., Seijo B., Remuñán-López C., The potential of chitosan for pulmonary drug delivery, Journal of Drug Delivery Science and Technology, 20, 1, 2010. Crossref
-
Xu Enyu, Jiang Wangzhan, Xu Ying, Cai Yang, Li Haoying, Pullulan-modified spray-dried powders for pulmonary drug delivery, Journal of Controlled Release, 213, 2015. Crossref
-
Yang Xiao-Fei, Xu Ying, Qu Da-Sheng, Li Hao-Ying, The influence of amino acids on aztreonam spray-dried powders for inhalation, Asian Journal of Pharmaceutical Sciences, 10, 6, 2015. Crossref
-
Mishima Kenji, Honjo Masatoshi, Sharmin Tanjina, Ito Shota, Kawakami Ryo, Kato Takafumi, Misumi Makoto, Suetsugu Tadashi, Orii Hideaki, Kawano Hiroyuki, Irie Keiichi, Sano Kazunori, Mishima Kenichi, Harada Takunori, Ouchi Mikio, Gas-saturated solution process to obtain microcomposite particles of alpha lipoic acid/hydrogenated colza oil in supercritical carbon dioxide, Pharmaceutical Development and Technology, 2015. Crossref
-
Pilcer Gabrielle, Wauthoz Nathalie, Amighi Karim, Lactose characteristics and the generation of the aerosol, Advanced Drug Delivery Reviews, 64, 3, 2012. Crossref
-
Li Hao-Ying, Seville Peter C., Novel pMDI formulations for pulmonary delivery of proteins, International Journal of Pharmaceutics, 385, 1-2, 2010. Crossref
-
Tewes F., Tajber L., Corrigan O.I., Ehrhardt C., Healy A.M., Development and characterisation of soluble polymeric particles for pulmonary peptide delivery, European Journal of Pharmaceutical Sciences, 41, 2, 2010. Crossref
-
Arora Sumit, Haghi Mehra, Young Paul M., Kappl Michael, Traini Daniela, Jain Sanyog, Highly respirable dry powder inhalable formulation of voriconazole with enhanced pulmonary bioavailability, Expert Opinion on Drug Delivery, 13, 2, 2016. Crossref
-
Colonna C., Conti B., Genta I., Alpar O.H., Non-viral dried powders for respiratory gene delivery prepared by cationic and chitosan loaded liposomes, International Journal of Pharmaceutics, 364, 1, 2008. Crossref
-
Liu Xiao-Bin, Ye Jun-Xiao, Quan Li-Hui, Liu Chun-Yu, Deng Xu-Liang, Yang Ming, Liao Yong-Hong, Pulmonary delivery of scutellarin solution and mucoadhesive particles in rats, European Journal of Pharmaceutics and Biopharmaceutics, 70, 3, 2008. Crossref
-
Minne Antoine, Boireau Hélène, Horta Maria Joao, Vanbever Rita, Optimization of the aerosolization properties of an inhalation dry powder based on selection of excipients, European Journal of Pharmaceutics and Biopharmaceutics, 70, 3, 2008. Crossref
-
Prota Lucia, Santoro Antonietta, Bifulco Maurizio, Aquino Rita P., Mencherini Teresa, Russo Paola, Leucine enhances aerosol performance of Naringin dry powder and its activity on cystic fibrosis airway epithelial cells, International Journal of Pharmaceutics, 412, 1-2, 2011. Crossref
-
Sosnowski Tomasz R., Gradoń Leon, Modification of inhalable powders by pulmonary surfactant components adsorbed on droplets during spray-drying process, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 365, 1-3, 2010. Crossref
-
Learoyd Tristan P., Burrows Jane L., French Eddie, Seville Peter C., Modified release of beclometasone dipropionate from chitosan-based spray-dried respirable powders, Powder Technology, 187, 3, 2008. Crossref
-
Jensen Ditte Marie Krohn, Cun Dongmei, Maltesen Morten Jonas, Frokjaer Sven, Nielsen Hanne Mørck, Foged Camilla, Spray drying of siRNA-containing PLGA nanoparticles intended for inhalation, Journal of Controlled Release, 142, 1, 2010. Crossref
-
Yang Feifei, Liu Xiaobin, Wang Wei, Liu Chunyu, Quan Lihui, Liao Yonghong, The effects of surface morphology on the aerosol performance of spray-dried particles within HFA 134a based metered dose formulations, Asian Journal of Pharmaceutical Sciences, 10, 6, 2015. Crossref
-
Wong Jennifer, Ricci Maurizio, Chan Hak-Kim, Spray Drying Strategies to Stop Tuberculosis, in Drug Delivery Systems for Tuberculosis Prevention and Treatment, 2016. Crossref
-
Alhusban Farhan A., Seville Peter C., Carbomer-modified spray-dried respirable powders for pulmonary delivery of salbutamol sulphate, Journal of Microencapsulation, 26, 5, 2009. Crossref
-
Costabile G., d'Angelo I., d'Emmanuele di Villa Bianca R., Mitidieri E., Pompili B., Del Porto P., Leoni L., Visca P., Miro A., Quaglia F., Imperi F., Sorrentino R., Ungaro F., Development of inhalable hyaluronan/mannitol composite dry powders for flucytosine repositioning in local therapy of lung infections, Journal of Controlled Release, 238, 2016. Crossref
-
Heng Desmond, Lee Sie Huey, Ng Wai Kiong, Tan Reginald BH, The nano spray dryer B-90, Expert Opinion on Drug Delivery, 8, 7, 2011. Crossref
-
Learoyd Tristan P., Burrows Jane L., French Eddie, Seville Peter C., Sustained delivery of salbutamol and beclometasone from spray-dried double emulsions, Journal of Microencapsulation, 27, 2, 2010. Crossref
-
Al-Khattawi Ali, Bayly Andrew, Phillips Andrew, Wilson David, The design and scale-up of spray dried particle delivery systems, Expert Opinion on Drug Delivery, 15, 1, 2018. Crossref
-
Lin Shan-Yang, Salmon calcitonin: conformational changes and stabilizer effects, AIMS Biophysics, 2, 4, 2015. Crossref
-
Ji Shuying, Thulstrup Peter Waaben, Mu Huiling, Hansen Steen Honoré, van de Weert Marco, Rantanen Jukka, Yang Mingshi, Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions, International Journal of Pharmaceutics, 534, 1-2, 2017. Crossref
-
Momin Mohammad A.M., Sinha Shubhra, Tucker Ian G., Doyle Colin, Das Shyamal C., Dry powder formulation of kanamycin with enhanced aerosolization efficiency for drug-resistant tuberculosis, International Journal of Pharmaceutics, 528, 1-2, 2017. Crossref
-
Akdag Cayli Yagmur, Sahin Selma, Buttini Francesca, Balducci Anna Giulia, Montanari Serena, Vural Imran, Oner Levent, Dry powders for the inhalation of ciprofloxacin or levofloxacin combined with a mucolytic agent for cystic fibrosis patients, Drug Development and Industrial Pharmacy, 43, 8, 2017. Crossref
-
Jiang Wang-Zhan, Cai Yang, Li Hao-Ying, Chitosan-based spray-dried mucoadhesive microspheres for sustained oromucosal drug delivery, Powder Technology, 312, 2017. Crossref
-
Al Zaitone Belal, Evaporation of oblate spheroidal droplets: A theoretical analysis, Chemical Engineering Communications, 205, 1, 2018. Crossref
-
Nikolaou Maria, Krasia-Christoforou Theodora, Electrohydrodynamic methods for the development of pulmonary drug delivery systems, European Journal of Pharmaceutical Sciences, 113, 2018. Crossref
-
Della Bella Andrea, Salomi Enrico, Buttini Francesca, Bettini Ruggero, The role of the solid state and physical properties of the carrier in adhesive mixtures for lung delivery, Expert Opinion on Drug Delivery, 15, 7, 2018. Crossref
-
Stevanović Magdalena, Polymeric micro- and nanoparticles for controlled and targeted drug delivery, in Nanostructures for Drug Delivery, 2017. Crossref
-
Zatta Kelly, Frank Luiza, Reolon Luciano, Amaral-Machado Lucas, Egito Eryvaldo, Gremião Maria, Pohlmann Adriana, Guterres Silvia, An Inhalable Powder Formulation Based on Micro- and Nanoparticles Containing 5-Fluorouracil for the Treatment of Metastatic Melanoma, Nanomaterials, 8, 2, 2018. Crossref
-
Razuc Mariela, Piña Juliana, Ramírez-Rigo María V., Optimization of Ciprofloxacin Hydrochloride Spray-Dried Microparticles for Pulmonary Delivery Using Design of Experiments, AAPS PharmSciTech, 19, 7, 2018. Crossref
-
Wang Xiaobo, Wang Yinan, Xi Ronggang, Wang Yuanyuan, Yang Xiaobo, Process optimization of spray-dried fanhuncaoin powder for pulmonary drug delivery and its pharmacokinetic evaluation in rats, Drug Development and Industrial Pharmacy, 44, 8, 2018. Crossref
-
Lu Wangding, Rades Thomas, Rantanen Jukka, Chan Hak-Kim, Yang Mingshi, Amino acids as stabilizers for spray-dried simvastatin powder for inhalation, International Journal of Pharmaceutics, 572, 2019. Crossref
-
Topal Gizem Rüya, Devrim Burcu, Eryilmaz Müjde, Bozkir Asuman, Design of ciprofloxacin-loaded nano-and microcomposite particles for dry powder inhaler formulations: preparation, in vitro characterisation, and antimicrobial efficacy, Journal of Microencapsulation, 35, 6, 2018. Crossref
-
Lin Yu, Chang Rachel Yoon Kyung, Britton Warwick J., Morales Sandra, Kutter Elizabeth, Li Jian, Chan Hak-Kim, Inhalable combination powder formulations of phage and ciprofloxacin for P. aeruginosa respiratory infections, European Journal of Pharmaceutics and Biopharmaceutics, 142, 2019. Crossref
-
Hickey Anthony J., Xu Zhen, Dry Powder Inhalers, in Particulate Products, 19, 2014. Crossref
-
Deshmukh Rohitas, Bandyopadhyay Nabamita, Abed Sara Nidal, Bandopadhyay Shantanu, Pal Yogendra, Deb Pran Kishore, Strategies for pulmonary delivery of drugs, in Drug Delivery Systems, 2020. Crossref
-
Wang Wenbo, Zhou Qi Tony, Sun Si-Ping, Denman John A., Gengenbach Thomas R., Barraud Nicolas, Rice Scott A., Li Jian, Yang Mingshi, Chan Hak-Kim, Effects of Surface Composition on the Aerosolisation and Dissolution of Inhaled Antibiotic Combination Powders Consisting of Colistin and Rifampicin, The AAPS Journal, 18, 2, 2016. Crossref
-
Ling Junhong, Mangal Sharad, Park Heejun, Wang Shaoning, Cavallaro Alex, Zhou Qi Tony, Simultaneous Particle Size Reduction and Homogeneous Mixing to Produce Combinational Powder Formulations for Inhalation by the Single-Step Co-Jet Milling, Journal of Pharmaceutical Sciences, 108, 9, 2019. Crossref
-
Xu Zhen, Hickey Anthony J., The Physics of Aerosol Droplet and Particle Generation from Inhalers, in Controlled Pulmonary Drug Delivery, 2011. Crossref
-
Bahr Mohamed M., Amer Mohamed S., Abo-El-Sooud Khaled, Abdallah Ahmed N., El-Tookhy Omar S., Preservation techniques of stem cells extracellular vesicles: a gate for manufacturing of clinical grade therapeutic extracellular vesicles and long-term clinical trials, International Journal of Veterinary Science and Medicine, 8, 1, 2020. Crossref
-
Ziaee Ahmad, Albadarin Ahmad B., Padrela Luis, Ung Mey-Tchieng, Femmer Tim, Walker Gavin, O'Reilly Emmet, A rational approach towards spray drying of biopharmaceuticals: The case of lysozyme, Powder Technology, 366, 2020. Crossref
-
Chow Michael Y.T., Chang Rachel Yoon Kyung, Chan Hak-Kim, Inhalation delivery technology for genome-editing of respiratory diseases, Advanced Drug Delivery Reviews, 168, 2021. Crossref
-
Douafer Hana, Andrieu Véronique, Brunel Jean Michel, Scope and limitations on aerosol drug delivery for the treatment of infectious respiratory diseases, Journal of Controlled Release, 325, 2020. Crossref
-
Chakraborty Amlan, Selomulya Cordelia, Formulation and role of polymeric and inorganic nanoparticles in respiratory diseases, in Targeting Chronic Inflammatory Lung Diseases Using Advanced Drug Delivery Systems, 2020. Crossref
-
P. A. Ferreira Mónica, Martins João Pedro, Hirvonen Jouni, Santos Hélder A., Spray-drying for the formulation of oral drug delivery systems, in Nanotechnology for Oral Drug Delivery, 2020. Crossref
-
Suzuki Érika Yoko, Simon Alice, da Silva Adriana Lopes, Amaro Maria Inês, de Almeida Gabriella Silva, Agra Laís Costa, Cabral Lucio Mendes, Rocco Patrícia Rieken Macedo, Healy Anne Marie, de Sousa Valeria Pereira, Effects of a novel roflumilast and formoterol fumarate dry powder inhaler formulation in experimental allergic asthma, International Journal of Pharmaceutics, 588, 2020. Crossref
-
Ding Li, Brunaugh Ashlee D., Stegemann Sven, Jermain Scott V., Herpin Matthew J., Kalafat Justin, Smyth Hugh D. C., A Quality by Design Framework for Capsule-Based Dry Powder Inhalers, Pharmaceutics, 13, 8, 2021. Crossref
-
Nikjoo Dariush, van der Zwaan Irès, Brülls Mikael, Tehler Ulrika, Frenning Göran, Hyaluronic Acid Hydrogels for Controlled Pulmonary Drug Delivery—A Particle Engineering Approach, Pharmaceutics, 13, 11, 2021. Crossref
-
Thanki Kaushik, Blum Kevin G, Thakur Aneesh, Rose Fabrice, Foged Camilla, Formulation of RNA interference-based drugs for pulmonary delivery: challenges and opportunities, Therapeutic Delivery, 9, 10, 2018. Crossref
-
Li Hao-Ying, Xu En-Yu, Innovative pMDI formulations of spray-dried nanoparticles for efficient pulmonary drug delivery, International Journal of Pharmaceutics, 530, 1-2, 2017. Crossref
-
AYDIN Özlem, ÜNLÜEL İsmigül, Enkapsülasyon Teknikleri ve Kontrollü Salım, European Journal of Science and Technology, 2022. Crossref
-
Shahin Hend, Vinjamuri Bhavani Prasad, Mahmoud Azza A., Mansour Suzan M., Chougule Mahavir Bhupal, Chablani Lipika, Formulation and optimization of sildenafil citrate-loaded PLGA large porous microparticles using spray freeze-drying technique: A factorial design and in-vivo pharmacokinetic study, International Journal of Pharmaceutics, 597, 2021. Crossref
-
Ziaee Ahmad, Albadarin Ahmad B., Padrela Luis, Femmer Tim, O'Reilly Emmet, Walker Gavin, Spray drying of pharmaceuticals and biopharmaceuticals: Critical parameters and experimental process optimization approaches, European Journal of Pharmaceutical Sciences, 127, 2019. Crossref
-
Al‐Qadi Sonia, Grenha Ana, Remuñán‐López Carmen, Chitosan‐Based Systems for Mucosal Delivery of Biopharmaceuticals, in Chitosan‐Based Systems for Biopharmaceuticals, 2012. Crossref
-
Ingvarsson Pall Thor, Schmidt Signe Tandrup, Christensen Dennis, Larsen Niels Bent, Hinrichs Wouter Leonardus Joseph, Andersen Peter, Rantanen Jukka, Nielsen Hanne Mørck, Yang Mingshi, Foged Camilla, Designing CAF-adjuvanted dry powder vaccines: Spray drying preserves the adjuvant activity of CAF01, Journal of Controlled Release, 167, 3, 2013. Crossref
-
Keil Tobias W.M., Feldmann Daniel P., Costabile Gabriella, Zhong Qian, da Rocha Sandro, Merkel Olivia M., Characterization of spray dried powders with nucleic acid-containing PEI nanoparticles, European Journal of Pharmaceutics and Biopharmaceutics, 143, 2019. Crossref
-
Natalini P.M., Razuc M.F., Sørli J.B., Bucalá V., Ramírez-Rigo M.V., The influence of surfactant on the properties of albendazole-bile salts particles designed for lung delivery, Journal of Drug Delivery Science and Technology, 53, 2019. Crossref
-
Agnoletti Monica, Bohr Adam, Thanki Kaushik, Wan Feng, Zeng Xianghui, Boetker Johan Peter, Yang Mingshi, Foged Camilla, Inhalable siRNA-loaded nano-embedded microparticles engineered using microfluidics and spray drying, European Journal of Pharmaceutics and Biopharmaceutics, 120, 2017. Crossref
-
Li L.J., Zhu Q.X., Gou D.Z., Chan H.-K., Kourmatzis A., Zhao G.T., Yang R.Y., DEM modelling of breakage behaviour of semi-brittle agglomerates subject to compaction and impaction, Powder Technology, 408, 2022. Crossref
-
Thakur Aneesh, Xu You, Cano-Garcia Guillermo, Feng Siqi, Rose Fabrice, Gerde Per, Andersen Peter, Christensen Dennis, Foged Camilla, Optimizing the design and dosing of dry powder inhaler formulations of the cationic liposome adjuvant CAF®01 for pulmonary immunization, Frontiers in Drug Delivery, 2, 2022. Crossref