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Critical Reviews™ in Biomedical Engineering
SJR: 0.207 SNIP: 0.376 CiteScore™: 0.79

ISSN Druckformat: 0278-940X
ISSN Online: 1943-619X

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Critical Reviews™ in Biomedical Engineering

DOI: 10.1615/CritRevBiomedEng.2014010461
pages 393-403

Recent Advances in Optical Microscopy Methods for Subcellular Imaging of Thick Biological Tissues

Nanguang Chen
Department of Biomedical Engineering, National University of Singapore, Singapore
Shakil Rehman
Department of Biomedical Engineering, National University of Singapore, Singapore
Colin J.R. Sheppard
Department of Biomedical Engineering, National University of Singapore, Singapore


Optical microscopy has been widely applied in cellular and subcellular imaging. Conventional light microscopes, however, have rather limited imaging depth and are limited to imaging only mechanically sectioned thin samples. Multiphoton microscopy and optical coherence microscopy are common techniques for diffraction-limited imaging beyond an imaging depth of 0.5 mm. Focal modulation microscopy is a novel method that combines confocal spatial filtering with focal modulation to reject out-of-focus backgrounds. Focal modulation microscopy has demonstrated an imaging depth comparable to those of multiphoton microscopy and optical coherence microscopy, near-real-time image acquisition, and capability with a multiple contrast mechanism.

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